Pparα

Constitutive Knock Out

Pparα Constitutive Knock Out Mouse Model
EZcohort™ Models
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C57BL/6 Background

  • Model #
  • Genotype
  • Nomenclature
  • 1640-F
    ko/ko
    B6.129S4-Pparatm1Gonz N12
  • 1640-M
    ko/ko
    B6.129S4-Pparatm1Gonz N12
  • Contains a disruption of the Pparα (peroxisome proliferator activated receptor alpha(α)) gene in the ligand-binding coding region
  • Pparα, a member of the nuclear steroid/hormone receptor superfamily, is involved in fatty acid metabolism and transport and mediates the induction of peroxisome proliferation
  • No profound hepatomegaly, peroxisomal proliferation and induction of fatty acid oxidation enzymes in response to classical peroxisome proliferators
  • Higher serum HDL cholesterol and apoA-I concentrations than wild type controls
  • β-oxidation of the long-chain fatty acid, palmitic acid, is lower in mutant mice, demonstrating altered mitochondrial fatty acid metabolism
  • β-oxidation of very long chain fatty acids is not altered
  • Useful in studies of lipid and glucose homeostasis, to study fatty acid metabolism and as a potential model for inborn errors of metabolism
Orders by weight: Taconic cannot accept orders by weight for this model. Please note that shipments may contain animals with a larger weight variation.

Genetic Background:

C57BL/6 Background

Origin:

The Pparα targeted mutation model was developed by Susana S.T. Lee and colleagues in the laboratory of Frank Gonzalez at the National Cancer Institute. The model was created by targeting the Pparα gene in 129S4 derived J1 ES cells and injecting the targeted cells into C57BL/6N blastocysts. Resultant chimeras were backcrossed to C57BL/6N for eleven generations (N11). Taconic received stock in 2001. Mice were backcrossed to C57BL/6N for embryo transfer derivation (N12). The colony is maintained by incrossing homozygous mice.


Genetics:

Wild type for Nnt mutation; carries Pde6brd1 mutation


Color:

Black

Species:

Mouse

Initial Publication:

Lee SS, Pineau T, Drago J, Lee EJ, Owens JW, Kroetz DL, Fernandez-Salguero PM, Westphal H, Gonzalez FJ. (1995) Targeted disruption of the a isoform of the peroxisome proliferator-activated receptor gene in mice results in abolishment of the pleiotropic effects of peroxisome proliferators. Mol Cell Biol, 15(5):3012-22.



Conditions of Use for EZcohort™ Models
EZcohort™ models are produced and distributed under rights to patents and intellectual property licensed from various institutions. Taconic Biosciences, Inc. (Taconic) sells EZcohort™ models to purchasers, grants to each purchaser a right under Taconic's rights in such licensed patents and intellectual property to use the purchased EZcohort™ models in consideration of purchasers' acknowledgement of and agreement to Taconic's Terms and Conditions for the Sale of Products and the following conditions of use:

  • Title to EZcohort™ models and biological materials derived from them remains with Taconic.
  • EZcohort™ models will be used for research purposes only.
  • EZcohort™ models and biological materials derived from them will not be distributed to third parties or used for commercial purposes.
  • Breeders from deliverables can be used for propagation of EZcohort™ models or to cross to other strains.
  • Continuation of breeding and crossbreeding of EZcohort™ models outside Taconicis permitted for a term of three years post-purchase of the EZcohort™ models and is subject to annual confirmation of these EZcohort™ Conditions of Use.

Continuation of breeding and crossbreeding after the three year term will require renewal of the EZcohort™ Conditions of Use and payment of a fee.