The Mdr1a/b-Bcrp mouse was developed in the laboratory of Alfred Schinkel of the Netherlands Cancer Institute. The model was created through crossbreeding of the Mdr1a/b targeted mutation mouse and the Bcrp targeted mutation mouse in the Schinkel lab. The Mdr1a/b model was created through sequential targeting of the Abcb1a and Abcb1b genes in E14 ES cells. Resultant chimeras were backcrossed to FVB/N for seven generations (N7). The Bcrp model was created by targeting the Abcg2 gene in E14 embryonic stem cells derived from 129P2/OlaHsd mice and injecting the targeted cells into FVB blastocysts. Resultant chimeras were backcrossed to FVB/N for seven generations (N7). Taconic received stock of the triple targeted mutation line in April 2005. The colony is maintained by mating animals homozygous for all three mutations.
Wild type for Nnt mutation; carries Pde6brd1 mutation
Jonker JW, Freeman J, Bolscher E, Musters S, Alvi AJ, Titley I, Schinkel AH, Dale TC (2005). Contribution of the ABC transporters Bcrp1 and Mdr1a/1b to the side population phenotype in mammary gland and bone marrow of mice. Stem Cells, 23(8):1059-1065.