Carries a transgene coding for the 695-amino acid isoform of human Alzheimer β-amyloid (Aβ) precursor protein carrying the Swedish mutation
Expresses high concentrations of the mutant Aβ, develops significant amyloid plaques and displays memory deficits
Useful for the study of APP expression, amyloid plaque formation, neuronal decline and memory loss associated with Alzheimer's disease (AD) as well as the study of drugs designed for treatment or prevention of Alzheimer's disease
NOTE: Premature mortality is an expected phenotype of this line, with mortality of 20% anticipated, particularly in males. Please take this into consideration when deciding upon order size to retain the needed experimental group size
Shipping Guidelines: Taconic recommends that customers purchase mice between 10 and 12 weeks of age. Due to a phenotype of sudden death at young ages, Taconic cannot guarantee shipment of mice 9 weeks of age or younger.
Pink eyed animals, associated with certain coat colors, and the Pde6brd1 retinal degeneration mutation can cause light sensitivity and/or blindness in some animals. This may impact the results of behavioral testing. Upon request, animals can be screened for eye color, coat color, and/or rd1 homozygosity for an additional fee
Rd1 tested mice (1349-RD1-F and 1349-RD1-M) are wild type or heterozygous for the Pde6brd1 mutation. Genotype shown in the table above refers to the APPSWE transgene.
Genetic Background: B6;SJL Mixed Background
The APPSWE mouse was developed in the laboratory of Karen Hsiao at the University of Minnesota, in association with the Mayo Clinic. This model was created by microinjecting the human APP695 gene containing the double mutation K670N, M671L into B6SJLF2 zygotes using a hamster prion protein cosmid vector. The resultant mice from Founder Line 2576 were backcrossed to C57BL/6. Taconic received stock from the Mayo Foundation in March 1999. Hemizygous males were backcrossed with C57BL/6NTac for derivation by embryo transfer. The colony is maintained by mating hemizygous male mice with B6SJLF1 female mice.
Availability: Ordering Note: Between 7 to 12 weeks of age males become aggressive and begin to fight. Male APPSWE mice are shipped in divided Taconic Transit Cages™ with one animal per section. We highly recommend housing APPSWE male mice one per cage.
Wild type for Nnt mutation, carries Pde6brd1 mutation
Hsaio K, Chapman P, Nilsen S, Eckman C, Harigaya Y, Younkin S, Yang F, Cole G. (1996) Correlative memory deficits, Aβ elevation, and amyloid plaques in transgenic mice. Science, 274:99-102.