NOD

Inbred

NOD Inbred Mouse Model

  • Model #
  • Genotype
  • Nomenclature
  • NOD-F
    NOD/MrkTac
  • NOD-M
    NOD/MrkTac
  • Exhibits destructive autoimmune pancreatic insulitis as early as four weeks of age
  • Insulin-dependent diabetes is found in some females beginning at three months of age and in approximately 60% of females and approximately 40% of males by six months of age

Origin:

The NOD inbred model was developed by intercrossing ICR mice. The mice were inbred twenty generations by Makino. Taconic received stock in 1985. The mice were derived by hysterectomy. The Taconic foundation colony was at F76 in 2005.


Genetics:

  • Strain Profile: Acy1s, Car2a, Ce2a, Es1b, Es3c, Es6a, Esda, Es11a, Fv1b, Ggca, Gpi1a Gus-sb, H6pd1b, Hbap, Hbbs, Idh1a, Ldh1a, Ldh2a, Ldr1a, Mod1b, Mpi1b, Mup1a, Pepcb, Pgdb, Pgm1a, Pgm2a, Pnpa, Sod1a, Trfb
  • Immunology: H2g7
  • Other: Ptprca

Color:

Albino

Species:

Mouse



n= 150 per sex at MPF, health standard from all global colonies. Data collected 2012-15.
High and Low represent mean +/- 2 standard deviations.
Based on sample size the charts above represents ~90% of the population.
All growth curves represent animals housed in our barriers, at our standard density and fed NIH31-M diet. Variations at customer facilities will alter expected growth curves.
Growth charts are provided only as a guide, if a specific weight criteria is needed please order animals by weight.

Customize this chart by clicking the legend elements, then explore download options by hovering your cursor over the down arrow to the right of the chart title.
Average litter size: 9
 
NOD Physiological Data Summary (July 2004; N=10/group):

Parameter Units NOD Males NOD Females
Serum Chemistry Avg ± S.D.    
Calcium mg/dL 10.6 ± 0.6 11.1 ± 0.3
Phosphorous mg/dL 11.8 ± 2.0 10.2 ± 3.6
Glucose mg/dL 116 ± 18 88 ± 17
Creatinine mg/dL 0.1 ± 0.0 0.1 ± 0.1
BUN mg/dL 22 ± 2 17 ± 6
Total Bilirubin mg/dL 0.2 ± 0.1 0.1 ± 0.0
ALK U/L 180 ± 11 184 ± 21
ALT U/L U/L 67 ± 63 44 ± 11
Total Protein g/dL 6.1 ± 0.6 6.2 ± 0.1
Blood Counts
Erythron      
Red Blood Cells X10^6/uL 8.54 ± 0.575 8.955 ± 0.476
Nucleated RBC X10^6/uL 0 ± 0 0 ± 0
Hemoglobin g/dL 15.2 ± 0.5 15.1 ± 0.7
Hematocrit % 46.7 ± 3.5 46.2 ± 2.5
MCV fL 55 ± 1 52 ± 0
MCH pG 17.8 ± 1.0 16.9 ± 0.2
MCHC % 32.7 ± 2.1 32.7 ± 0.4
Platelets      
Platelets X10^3/uL 1165 ± 334 1127 ± 291
Leukogram      
White Blood Cells x10^3/uL 3.654 ± 0.875 5.438 ± 1.234
Neutrophil x10^3/uL 1.211 ± 0.326 2.011 ± 0.872
Bands x10^3/uL 0 ± 0 0 ± 0
Lymphocyte x10^3/uL 2.400 ± 0.999 3.380 ± 1.139
Monocytes x10^3/uL 0.039 ± 0.068 0.016 ± 0.030
Eosinophil x10^3/uL 0.003 ± 0.010 0.031 ± 0.063
Basophils x10^3/uL 0 ± 0 0 ± 0
Others x10^3/uL 0 ± 0 0 ± 0
Organ Weights      
Stomach g 0.275 ± 0.016 0.257 ± 0.025
Ileum g 0.791 ± 0.062 0.799 ± 0.098
Colon g 0.206 ± 0.042 0.192 ± 0.050
Lungs g 0.228 ± 0.024 0.226 ± 0.025
Heart g 0.174 ± 0.032 0.136 ± 0.017
Liver g 1.139 ± 0.072 0.955 ± 0.076
Spleen/Pancreas g 0.228 ± 0.032 0.193 ± 0.027
Kidney (L) g 0.204 ± 0.017 0.137 ± 0.011
Kidney (R) g 0.204 ± 0.017 0.138 ± 0.013
Testes (L) g 0.103 ± 0.008 -
Testes (R) g 0.189 ± 0.268 -
Ovary (L) g - 0.020 ± 0.008
Ovary (R) g - 0.021 ± 0.012
Urinalysis      
Glucose mg/dL Negative Negative
Blood - Negative Negative
PH - 6.85 ± 0.41 6.45 ± 0.16
Protein mg/dL Negative Negative
Specific Gravity - 1.01 ± 0.01 1.02 ± 0.00

Phenotyping Procedures:


Animal Receipt and Maintenance

A quantity of twenty mice (ten males and ten females) was submitted for testing, strain identified as Line NOD (NOD/MrkTac), age 7 weeks. Mice were received at RBU 3, Taconic Biotechnology, Albany NY and acclimated for three days on irradiated NIH31 diet and sterilized water ad lib, sterile contact bedding (paper chip) and a 12:12 light:dark cycle. All animals appeared normal during this period and routine health surveillance of this colony detected no microbial pathogens. Mice were assigned study unique identification numbers (males 1-10, females 11-20).

 

Urinalysis

All mice were fasted in metabolic cages and an overnight urine sample was collected. Urine analysis was performed using Multistix 10 SG (Bayer). Strips were read and recorded as per the manufacturer's instructions, and the results are presented in Table 2.

 

Clinical Chemistry and Hematology

A terminal blood sample was taken from Carbon Dioxide - anesthetized mice via cardiac puncture. The collected blood was divided into two samples. One sample was treated with EDTA and stored at 4°C for hematological evaluation. Another sample was allowed to clot at 4°C for 30 minutes, and then centrifuged at 7000rpm for 10 minutes, and the serum was decanted and frozen at -80°C for clinical chemistry analysis. A slide smear was made from a single drop of whole blood. Frozen serum, chilled whole blood and slides were delivered to LabCorp (RTP, NC) for analysis; the results are presented in Table 3. Unless otherwise indicated, serum chemistry data is generated from a Hitachi 717 automated analyzer and hematological data is generated from a Celldyne 3500. WBC differential counts are performed manually.

 

Necropsy and Organ Weights

All mice were euthanized and bodyweights were recorded. Representative tissues were collected, weighed, and immersion fixed in 10% Neutral Buffered Formalin. Tissues were delivered to the Taconic lab for histological preparation and evaluation. Tabulation of organ weights is presented in Table 4. The pathologist's summary and detailed histological descriptions follow.

 

Discussion

All animals were thrifty on arrival and appeared clinically normal. Locomotor behavior was normal and there were no visible lesions or discharges at the mucous membranes. All tissues appeared normal on gross necropsy evaluation and weights were within normal limits. There were no unusual findings upon collection of these tissues nor otherwise observed in the body cavities.

The overall profile of these mice is consistent with expectations of a general-purpose mouse. None of the parameters are indicative of impaired system function.