ICR

Outbred

ICR Outbred Mouse Model
Available for immediate cryorecovery

  • Model #
  • Genotype
  • Nomenclature
  • ICR-F
    IcrTac:ICR
  • ICR-M
    IcrTac:ICR
  • Good reproductive performance and fast growth rate
  • Often used as an embryo donor and/or recipient mother in transgenic mouse labs
  • It has been used extensively in toxicology and pharmacology studies and is often used for product safety testing

Origin:

The ICR outbred model was developed by Dr. T. S. Hauschka of Fox Chase Cancer Center in 1948. Dr. Hauschka left Fox Chase a portion of his colony. Taconic received breeder stock from Fox Chase in 1993. The mice were derived by hysterectomy into IBU colonies and are maintained by Poiley rotational bred mice.


Availability:

Available for immediate cryorecovery

Genetics:

Taconic's outbred colony is known to carry the recessive mutation, Pde6brd1


Coat Color Loci:

Tyrc

Color:

Albino

Species:

Mouse



n= 120 per sex at MPF health standard from US production colonies. Data collected 2011-13.
High and Low represent mean +/- 2 standard deviations.
Based on sample size the charts above represents ~75% of the population.
All growth curves represent animals housed in our barriers, at our standard density and fed NIH31-M diet. Variations at customer facilities will alter expected growth curves.
Growth charts are provided only as a guide, if a specific weight criteria is needed please order animals by weight.

Customize this chart by clicking the legend elements, then explore download options by hovering your cursor over the down arrow to the right of the chart title.
ICR Physiological Data Summary (January 2003; N=10/group):

Parameter Units ICR Males ICR Females
Serum Chemistry Avg ± S.D.    
Calcium mg/dL 10.6 ± 0.3 10.5 ± 0.4
Phosphorous mg/dL 11.1 ± 1.1 9.2 ± 0.9
Glucose mg/dL 181 ± 44 114 ± 18
Creatinine mg/dL 0.1 ± 0.1 0.1 ± 0.0
BUN mg/dL 25 ± 6 19 ± 1
Total Bilirubin mg/dL 0.2 ± 0.0 0.1 ± 0.0
ALK U/L 109 ± 15 145 ± 21
ALT U/L U/L 30 ± 9 39 ± 16
Total Protein g/dL 5.9 ± 0.3 6.3 ± 0.3
Blood Counts
Erythron      
Red Blood Cells X10^6/uL 9.9525 ± 0.4818 9.5422 ± 0.6053
Nucleated RBC X10^6/uL 0 ± 0 0 ± 0
Hemoglobin g/dL 15.9 ± 0.5 16.0 ± 0.7
Hematocrit % 52.0 ± 2.6 50.6 ± 2.5
MCV fL 52 ± 2 53 ± 1
MCH pG 16.0 ± 0.3 16.8 ± 0.7
MCHC % 30.5 ± 0.7 31.6 ± 1.2
Platelets      
Platelets X10^3/uL 1374 ± 257 1072 ± 162
Leukogram      
White Blood Cells x10^3/uL 6.6625 ± 2.0486 5.7777 ± 1.8329
Neutrophil x10^3/uL 0.954 ± 0.705 0.779 ± 0.232
Bands x10^3/uL 0 ± 0 0 ± 0
Lymphocyte x10^3/uL 5.523 ± 1.710 4.792 ± 1.606
Monocytes x10^3/uL 0.156 ± 0.142 0.148 ± 0.087
Eosinophil x10^3/uL 0.030 ± 0.042 0.058 ± 0.062
Basophils x10^3/uL 0 ± 0 0 ± 0
Others x10^3/uL 0 ± 0 0 ± 0
Organ Weights      
Stomach g 0.317 ± 0.045 0.319 ± 0.038
Ileum g 1.060 ± 0.211 0.915 ± 0.088
Colon g 0.278 ± 0.067 0.286 ± 0.054
Lungs g 0.308 ± 0.057 0.269 ± 0.044
Heart g 0.209 ± 0.042 0.160 ± 0.017
Liver g 1.636 ± 0.241 1.247 ± 0.091
Spleen/Pancreas g 0.293 ± 0.046 0.275 ± 0.039
Kidney (L) g 0.277 ± 0.060 0.183 ± 0.013
Kidney (R) g 0.289 ± 0.060 0.178 ± 0.018
Testes (L) g 0.114 ± 0.023 -
Testes (R) g 0.115 ± 0.020 -
Ovary (L) g - 0.019 ± 0.005
Ovary (R) g - 0.017 ± 0.009
Urinalysis      
Glucose mg/dL 29.67 ± 3.70 23.08 ± 1.22
Blood - Negative Negative
Ph - 6.45 ± 0.28 6.30 ± 0.26
Protein mg/dL Trace/30+ Trace
Specific Gravity - 1.02 ± 0 1.02 ± 0.01

Phenotyping Procedures:

Animal Receipt and Maintenance

A quantity of twenty mice (ten males and ten females) was submitted for testing, strain identified as Line ICR (IcrTac:ICR), age 7 weeks. Mice were received at RBU 3, Taconic Biotechnology, Albany NY and acclimated for three days on irradiated NIH #31M rodent diet and sterilized water ad lib, sterile contact bedding (paper chip) and a 12:12 light:dark cycle. All animals appeared normal during this period and routine health surveillance of this colony detected no microbial pathogens. Mice were assigned study unique identification numbers (males 1-10, females 11-20).

 

Urinalysis

All mice were fasted in metabolic cages and an overnight urine sample was collected. Urine analysis was performed using Multistix 10 SG (Bayer). Strips were read and recorded as per the manufacturer's instructions.

 

Clinical Chemistry and Hematology

A terminal blood sample was taken from Carbon Dioxide - anesthetized mice via cardiac puncture. The collected blood was divided into two samples. One sample was treated with EDTA and stored at 4°C for hematological evaluation. Another sample was allowed to clot at 4°C for 30 minutes, and then centrifuged at 7000rpm for 10 minutes, and the serum was decanted and frozen at -80°C for clinical chemistry analysis. A slide smear was made from a single drop of whole blood. Frozen serum, chilled whole blood, and slides were delivered to LabCorp (RTP, NC) for analysis. Unless otherwise indicated, serum chemistry data is generated from a Hitachi 717 automated analyzer and hematological data is generated from a Celldyne 3500. WBC differential counts are performed manually.

 

Necropsy and Organ Weights

All mice were euthanized and bodyweights were recorded. Representative tissues were collected, weighed, and immersion fixed in 10% Neutral Buffered Formalin. Tissues were delivered to the Taconic lab for histological preparation and evaluation. The pathologist's summary and detailed histological descriptions follow.

 

Discussion

All animals were thrifty on arrival and appeared clinically normal. Locomotor behavior was normal and there were no visible lesions or discharges at the mucous membranes. All tissues appeared normal on gross necropsy evaluation and weights were within normal limits. There were no unusual findings upon collection of these tissues nor otherwise observed in the body cavities.

The overall profile of these mice is consistent with expectations of a general-purpose mouse. None of the parameters are indicative of impaired system function.