huNOG-EXL

NOD Background

  • Model #
  • Genotype
  • Nomenclature
  • HSCFTL-13395-F
    sp/sp;ko/ko;tg/wt
    NOD.Cg-Prkdcscid Il2rgtm1Sug Tg(SV40/HTLV-IL3,CSF2)10-7Jic/JicTac
  • HSCCB-13395-F
    sp/sp;ko/ko;tg/wt
    NOD.Cg-Prkdcscid Il2rgtm1Sug Tg(SV40/HTLV-IL3,CSF2)10-7Jic/JicTac
  • Super immunodeficient NOG mouse expressing human GM-CSF and human IL-3 cytokines to support myeloid lineage engraftment
  • Higher overall engraftment levels of human hematopoietic stem cells (HSC) compared to core NOG mouse
  • Higher levels of myeloid cell differentiation following human HSC engraftment compared to core NOG mouse
  • Model of human allergy response
  • Applications in research involving cancer, infectious disease, immunology, regenerative medicine and humanization

Origin:

hGM-CSF/hIL3 NOG mice engrafted with human CD34+ hematopoietic stem cells (HSCs) stably develop extensive cell lineages as early as 6 to 8weeks post-injection. Both myeloid and lymphoid lineage cells are present in peripheral blood, bone marrow, thymus and spleen and non-lymphoid tissue including lung and liver. Longer term studies are possible in huNOG-EXL mice, with literature reports of stable engraftment and hematopoiesis for at least 20 weeks post engraftment. While, not all human immune cell types have been exhaustively characterized in these mice, extensive myeloid differentiation including granulocyte differentiation (basophil, neutrophil and mast cells) are evident in blood and tissues.Antigen presenting cells (dendritic cells and macrophages) are also increased in frequency, both in blood and spleen.


Availability:

Taconic maintains an inventory of female huNOG-EXL mice engrafted as juveniles and FACS tested at 12 weeks post-engraftment; in order to ship, all mice must be ≥25% hCD45+ in peripheral blood, but in most cases huNOG-EXL mice average > 40% human cells in blood. Custom options for huNOG-EXL generation are available, such as HLA selection and age of QC and delivery post engraftment. For a limited time, Taconic Biosciences is offering a trial program to enable you to validate the huNOG model in your research facility.

Color:

Albino

Species:

Mouse

Initial Publication:

Ito M, Hiramatsu H, Kobayashi K, Suzue K, Kawahata M, Hioki K, Ueyama Y, Koyanagi Y, Sugamura K, Tsuji K, Heike T, Nakahata T. (2002) NOD/SCID/γnull/c mouse: an excellent recipient mouse model for engraftment of human cells. Blood 100(9):3175-3182.

Fukuchi Y, Miyakawa Y, Kobayashi K, Kuramochi T, Shimamura K, Tamaoki N, Nomura T, Ueyama Y, Ito M. (1998) Cytokine dependent growth of human TF-1 leukemic cell line in human GM-CSF and IL-3 producing transgenic SCID mice. Leuk Res 22(9):837-43.

Ito R, Takahashi T, Katano I, Kawai K, Kamisako T, Ogura T, Ida-Tanaka M, Suemizu H, Nunomura S, Ra C, Mori A, Aiso S, Ito M. (2013) Establishment of a human allergy model using human IL-3/GM-CSF-transgenic NOG mice. J Immunol.191(6):2890-9.