Flp Deleter

Random Transgenic

Flp Deleter Random Transgenic Mouse Model

C57BL/6 Background

  • Model #
  • Genotype
  • Nomenclature
  • 7089-F
    tg/wt
    C57BL/6-Tg(CAG-Flpe)2Arte
  • 7089-M
    tg/wt
    C57BL/6-Tg(CAG-Flpe)2Arte
  • 7089-F
    tg/tg
    C57BL/6-Tg(CAG-Flpe)2Arte
  • 7089-M
    tg/tg
    C57BL/6-Tg(CAG-Flpe)2Arte
  • Ubiquitously active general Flp deleter.
  • In vitro-derived Flp recombinase under the control of the chicken beta-actin promoter and an hCMV immediate early enhancer. The use of the chimeric CMV enhancer/beta-actin promoter leads to a ubiquitous expression profile in eukaryotes. The Flp recombinase carries a minimal, nuclear localization signal from the SV40 larger T-antigen. Additional elements of the expression cassette are an intron with splice donor and acceptor sites upstream of the Flp gene and a synthetic polyadenylation signal downstream of the Flp.
  • This colony is currently being intracrossed to homozygosity. In the interim, mice may be sold as carriers. Mice with a genotype of Carrier(C) carry the transgene, but may be either hemizygous or homozygous.

Genetic Background:

C57BL/6 Background

Origin:

The Flp Deleter mouse was developed by Artemis Pharmaceuticals (now Taconic). The mutation was introduced through random integration (microinjection) of the CAGGS-Flpe into C57BL/6 zygotes. The line was backcrossed 10 generations to C57BL/6NTac and intercrossed to homozygosity. The line was rederived in 2013 and is currently being intracrossed to homozygosity.


Color:

Black

Species:

Mouse


Access to this model requires execution of a Flp Deleter Research Crossbreeding Agreement. Current agreement fees for this model are $1,500/year (€1,125/year) for nonprofit users and $6,000/year (€4,500/year) for for-profit users. Nonprofit transgenic cores should inquire for pricing and terms. Once the agreement is executed, you may order mice for use in crossbreeding. For general inquiries, please contact Taconic Customer Service. To request an agreement, please inquire.