CIEA BRG mouse

Constitutive Knock Out

CIEA BRG Constitutive Knock Out Mouse Model

BALB/c Background

  • Model #
  • Genotype
  • Nomenclature
  • 11503-F
    C.Cg-Rag2tm1Fwa Il2rgtm1Sug/JicTac
  • 11503-M
    C.Cg-Rag2tm1Fwa Il2rgtm1Sug/JicTac
  • Immunodeficient model lacking mature T, B, and NK cells
  • Produces no immunoglobulins; has dysfunction dendritic cells
  • No incidence of T or B cell leakiness
  • Higher radiation tolerance due to Rag2 mutation compared to scid models (similar to Wild Type mice)
  • Model is completely congenic on BALB/c background, the preferred strain background for many immunology studies

Genetic Background:

BALB/c Background


The BRG mouse was developed by Mamoru Ito of the Central Institute for Experimental Animals (CIEA) in Japan. The Rag2 mouse was developed in the laboratory of Frederick W. Alt at Columbia University. The Rag2 targeted mutation was created by targeting the Rag2 gene in CCE ES cells derived from the 129S6 strain. Targeted ES cells were injected into C57BL/6 or MF1 blastocysts. The Il2rg targeted mutation was developed by Dr. Kazuo Sugamura of Tohoku University by targeting the gene in El4-1 or CCE ES cells derived from a 129 strain. Targeted ES cells were injected into C57BL/6 blastocysts. CIEA received the mouse as a non-inbred strain. The Rag2 mutation was backcrossed at CIEA to BALB/cA for at least 8 generations to generate BALB/cA-Rag2tm1Fwa. The mouse carrying Il2rgtm1Sug on a non-inbred strain background was then backcrossed to BALB/cA-Rag2tm1Fwa for eight generations to generate the BRG mouse. Taconic received stock in 2011, and the line was derived through embryo transfer. The mice are maintained by breeding females homozygous for both the Rag2 and Il2rg mutant alleles with males that are homozygous for the Rag2 allele and hemizygous for the Il2rg mutant allele.


Model Sponsor: The Central Institute for Experimental Animals and In-Vivo Science International





Initial Publication:

Traggiai E, Chicha L, Mazzucchelli L, Bronz L, Piffaretti JC, Lanzavecchia A, Manz MG. (2004) Development of a human adaptive immune system in cord blood cell-transplanted mice. Science. 304(5667):104-7.

Nonprofit users (excluding users at nonprofit foundations which are affiliated with a for-profit entity):

For internal research purposes, the CIEA BRG mouse Conditions of Use for nonprofit users apply. If you wish to perform sponsored research or fee-for-service contract research using the CIEA BRG mouse, please inquire for access conditions.

For-profit users and users at foundations which are affiliated with for-profit entities:

The CIEA BRG mouse Conditions of Use for for-profit users apply.

Does your research require the use of neonates? Please inquire for more information on neonate access options.