129S6

Inbred

129S6 Inbred Mouse Model

  • Model #
  • Genotype
  • Nomenclature
  • 129SVE-F
    129S6/SvEvTac
  • 129SVE-M
    129S6/SvEvTac
  • Progenitors of this strain used extensively to establish Embryonic Stem (ES) cell lines (e.g., W4) for the production of targeted mutation (knockout) mice
  • Targeted mutation mice developed from 129 agouti ES cell lines can often be maintained with maximum genomic homozygosity by mating chimeras to the 129S6 strain
  • Taconic's 129 mice are of the Steel ('S') subline.

Origin:

Taconic received 129S6 inbred model stock from GenPharm International from two sublines originating from Dr. Martin Evan's colony in 1992. The mice were derived by embryo transfer in 1993. The resulting offspring were heterozygous at Gpi1 for the 'a' and 'c' alleles. Homozygous Gpi-1c brother x sister mice were selected in the Foundation Colony at F8 and became the common ancestors for all subsequent Taconic breeders. The Taconic foundation colony was at F42 in 2005.


Availability:

Live production in Denmark of this model ceased on November 27, 2015. This model continues to be available from live colonies in the US.

Genetics:

  • Strain Profile: Acy1s, Akp1b, Amy1a, Apoa1b, Car2a, Es1b, Es3c, Esdb, Es11a, Ggca, Glo1a, Got2b, Gpi1c, Gpt1a, Gr1a, Gus-sb, H6pdb, Hbbd, Idh1a, Ldr1a, Mod1a, Mpi1b, Neu1b, Pep3b, Pgm1b, Pgm2a, Pnpa, Trfb, Sod1a
  • Immunology: H2b
  • Other: Ptprcb

Coat Color Loci:

Aw

Species:

Mouse

Initial Publication:

Simpson, EM, et. al; (1997) "Genetic Variation Among 129 substrains and its Importance for 'Targeted Mutagenesis' in Mice." Nature Genetics; Vol. 16(1), p. 19



For some applications, weight may be critical. For orders where weight is critical, please place orders by weight, not by age. Taconic can accept orders by weight for this model. A minimum 3 gram span is required. All weights are weights at time of packing. Mice and rats can lose weight in transit. Orders for specific weight spans will be assessed a nominal fee. More detail on weight order policies is available.

n = 250 per sex at MPF and RF health standards from all global colonies.
High and Low represent mean +/- 2 standard deviations.
Based on sample size the charts above represent ~90% of the population.
All growth curves represent animals housed in our barriers, at our standard density and fed NIH31-M diet. Variations at customer facilities will alter expected growth curves.
Growth charts are provided only as a guide, if a specific weight criteria is needed please order animals by weight.

Customize this chart by clicking the legend elements, then explore download options by hovering your cursor over the down arrow to the right of the chart title.
Average litter size: 8

Phenotyping Procedures:


129SVE Physiological Data Summary (May 2003; N=10/group):
Parameter Units 129SVE Males 129SVE Females
Serum Chemistry Avg ± S.D.    
Calcium mg/dL 11.8 ± 0.5 10.7 ± 0.6
Phosphorous mg/dL 11.9 ± 1.9 9.9 ± 1.3
Glucose mg/dL 120 ± 23 98 ± 18
Creatinine mg/dL 0.2 ± 0.1 0.1 ± 0.0
BUN mg/dL 31 ± 10 20 ± 2
Total Bilirubin mg/dL 0.1 ± 0.1 0.1 ± 0.0
ALK U/L 199 ± 34 233 ± 25
ALT U/L U/L 19 ± 5 36 ± 38
Total Protein g/dL 5.8 ± 0.2 5.3 ± 0.2
Blood Counts
Erythron      
Red Blood Cells X10^6/uL 10.043 ± 0.335 9.4725 ± 0.6297
Nucleated RBC X10^6/uL 0 ± 0 0 ± 0
Hemoglobin g/dL 16.7 ± 0.5 15.9 ± 0.7
Hematocrit % 52.9 ± 1.5 49.0 ± 2.1
MCV fL 53 ± 1 52 ± 2
MCH pG 16.7 ± 0.2 16.8 ± 0.5
MCHC % 31.6 ± 0.3 32.4 ± 0.7
Platelets      
Platelets X10^3/uL 799 ± 97 765 ± 59
Leukogram      
White Blood Cells x10^3/uL 5.93 ± 0.60 5.30875 ± 1.22240
Neutrophil x10^3/uL 0.571 ± 0.285 0.639 ± 0.307
Bands x10^3/uL 0 ± 0 0 ± 0
Lymphocyte x10^3/uL 5.174 ± 0.541 4.473 ± 1.131
Monocytes x10^3/uL 0.168 ± 0.088 0.124 ± 0.085
Eosinophil x10^3/uL 0.012 ± 0.025 0.073 ± 0.090
Basophils x10^3/uL 0.0062 ± 0.0196 0 ± 0
Others x10^3/uL 0 ± 0 0 ± 0
Organ Weights      
Stomach g 0.236 ± 0.038 0.22 ± 0.03
Ileum g 0.630 ± 0.064 0.59 ± 0.13
Colon g 0.127 ± 0.027 0.13 ± 0.02
Lungs g 0.227 ± 0.024 0.19 ± 0.02
Heart g 0.156 ± 0.026 0.12 ± 0.01
Liver g 0.859 ± 0.064 0.70 ± 0.11
Spleen/Pancreas g 0.199 ± 0.029 0.17 ± 0.02
Kidney (L) g 0.164 ± 0.017 0.11 ± 0.01
Kidney (R) g 0.154 ± 0.012 0.11 ± 0.01
Testes (L) g 0.091 ± 0.025 -
Testes (R) g 0.101 ± 0.018 -
Ovary (L) g - 0.01 ± 0.01
Ovary (R) g - 0.02 ± 0.03
Urinalysis      
Glucose mg/dL Neg. Neg.
Blood - Neg. Neg.
Ph - 6.3 ± 0.3 6.4 ± 0.2
Protein mg/dL Trace Trace
Specific Gravity - 1.026 ± 0.004 1.016 ± 0.008

Animal Receipt and Maintenance

A quantity of twenty mice (ten males and ten females) was submitted for testing, strain identified as Line 129SVE (129S6/SvEvTac), age 7 weeks. Mice were received at RBU 3, Taconic Biotechnology, Albany NY and acclimated for three days on irradiated NIH #31M rodent diet and sterilized water ad lib, sterile contact bedding (paper chip) and a 12:12 light:dark cycle. All animals appeared normal during this period and routine health surveillance of this colony detected no microbial pathogens. Mice were assigned study unique identification numbers (males 1-10, females 11-20).

 

Urinalysis

All mice were fasted in metabolic cages and an overnight urine sample was collected. Urine analysis was performed using Multistix 10 SG (Bayer). Strips were read and recorded as per the manufacturer's instructions, and the results are presented in Table 1.

 

Clinical Chemistry and Hematology

A terminal blood sample was taken from Carbon Dioxide - anesthetized mice via cardiac puncture. The collected blood was divided into two samples. One sample was treated with EDTA and stored at 4°C for hematological evaluation. Another sample was allowed to clot at 4°C for 30 minutes, and then centrifuged at 7000rpm for 10 minutes, and the serum was decanted and frozen at -80°C for clinical chemistry analysis. A slide smear was made from a single drop of whole blood. Frozen serum, chilled whole blood, and slides were delivered to LabCorp (RTP, NC) for analysis; the results are presented in Table 2. Unless otherwise indicated, serum chemistry data is generated from a Hitachi 717 automated analyzer and hematological data is generated from a Celldyne 3500. WBC differential counts are performed manually. No blood samples were obtained from mouse #16.

 

Necropsy and Organ Weights

All mice were euthanized and bodyweights were recorded. Representative tissues were collected, weighed, and immersion fixed in 10% Neutral Buffered Formalin. Tissues were delivered to the Taconic lab for histological preparation and evaluation. Tabulation of organ weights is presented in Table 3. The pathologist's summary and detailed histological descriptions follow.


Model 129SVE (strain: 129S6/SvEvTac) Reproductive Data:

Age at Breeder Setup (Sexual Maturity): 7-8 weeks
Summary: The Average Female Breeder produces a litter of 6.4 pups every 5.4 weeks
Criteria Average Std.Dev Low High N=
Newborn Litter Size 6.3 0.88 1 11 112
Weaned Litter Size 6.4 0.93 2 11 107
Weeks from Setup to 1st Litter 3.7 0.5 3 4 25
# of Weeks Breeders Productive 2) 23 8 5 34 25

Features % N=
% of Breeder Pairs Not Producing A Litter (i.e. possible sterility) 0.0% 25 pairs
% of Entire Litters Cannibalized 5.1% 118 litters
Newborn Mortality (excluding Entire Litter Cannibalism) 3.1% 709 newborns
Females as % of Total Weanlings (Sex Ratio) 54% 687 weanlings
Active Breeder Production Efficiency Index (PEI) 1.19 576 Breeder Weeks
1st Litter - Entire Litter Cannibalism Rate 4.0% 25 litters
1st Litter - Newborn Mortality (excluding Entire Litter Cannibalism) 7.7% 143 newborns
Footnotes:
  1. Criteria for Retiring an Active Breeder: No litter or pregnancy observed within six weeks of breeder setup date. Breeder has two consecutive lost litters. Breeder has two consecutive litters of less than 2-3 pups. Breeder found moribund. Breeders generally scheduled for teardown after 30-34 weeks of active breeding
  2. Maximum allowed breeder age in Taconic's commercial Production colonies is generally 30-34 weeks