Orders by weight:
Contains a disruption of the recombination activating gene 2 (Rag2)
Homozygous mice exhibit total inability to initiate V(D)J rearrangement and fail to generate mature T or B lymphocytes. Otherwise, the Rag2 mouse has apparently normal hematopoiesis
Useful for vaccine development, transplantation studies and hematopoiesis research. Through blastocyst complementation assays, can be useful in evaluating the function of specific genes in the differentiation of lymphocytes
Carries the H2b haplotype
Taconic cannot accept orders by weight for this model. Please note that shipments may contain animals with a larger weight variation.
Genetic Background: C57BL/6 Background
Origin: The Rag2 mouse was developed in the laboratory of Frederick W. Alt at Columbia University. The model was created by targeting the Rag2 gene in CCE ES cells and injecting the targeted cells into blastocysts. This line originated from model RAG2-M on a 129S6/SvEv background. The mice were backcrossed twelve generations (N12) to a C57BL/6NTac inbred background. Heterozygotes were intercrossed to generate homozygous targeted mutation mice. The line is maintained by incrossing homozygous mice.
Genetics: Wild type for Nnt mutation
Shinkai, Y, Rathbun, G, Lam, KP, Oltz, EM, Stewart, V, Mendelsohn, M, Charron, J, Datta, M, Young, F, Stall, AM, and Alt, FW. (1992) RAG-2-Deficient Mice Lack Mature Lymphocytes Owing to Inability to Initiate V(D)J Rearrangement
, Cell, 68: 855-867