Contains a disruption of the recombination activating gene 2 (Rag2)
Homozygous mice exhibit total inability to initiate V(D)J rearrangement and fail to generate mature T or B lymphocytes. Otherwise, the Rag2 mouse has apparently normal hematopoiesis
Useful for vaccine development, transplantation studies and hematopoiesis research. Through blastocyst complementation assays, can be useful in evaluating the function of specific genes in the differentiation of lymphocytes
Carries the H2b haplotype
Taconic recommends use of RAGN12 mice at the Murine Pathogen Free (MPF) for colitis studies involving adoptive T cell transfer. Use of RAGN12 mice at the Opportunist Free (OF) health standard should be considered based on facility-specific bioexclusion needs and other experimental considerations.
Orders by weight: Taconic cannot accept orders by weight for this model. Please note that shipments may contain animals with a larger weight variation.
RAGN12 has new data for the adoptive transfer colitis model.
The Rag2 mouse was developed in the laboratory of Frederick W. Alt at Columbia University. The model was created by targeting the Rag2 gene in CCE ES cells and injecting the targeted cells into blastocysts. This line originated from model RAG2-M on a 129S6/SvEv background. The mice were backcrossed twelve generations (N12) to a C57BL/6NTac inbred background. Heterozygotes were intercrossed to generate homozygous targeted mutation mice. The line is maintained by incrossing homozygous mice.
Genetics:
Wild type for Nnt mutation
Color:
Black
Species:
Mouse
Initial Publication:
Shinkai, Y, Rathbun, G, Lam, KP, Oltz, EM, Stewart, V, Mendelsohn, M, Charron, J, Datta, M, Young, F, Stall, AM, and Alt, FW. (1992) RAG-2-Deficient Mice Lack Mature Lymphocytes Owing to Inability to Initiate V(D)J Rearrangement, Cell, 68: 855-867.
Conditions of Use for Taconic Transgenic Models™
Taconic Transgenic Models™ (Models) are produced and distributed under rights to patents and intellectual
property licensed from various institutions. Taconic sells the Models to purchasers, grants to each purchaser a
right under Taconic's rights in such licensed patents and intellectual property to use the purchased Model in
consideration of purchasers' acknowledgement of and agreement to the
Terms and Conditions for Taconic Models, Products and Services
and the following terms of use:
Title to these Models and biological materials derived from them remains with Taconic.
The Models will be used for research purposes only.
The Models will not be bred or cross-bred except to obtain embryos or fetuses required for research purposes unless additional rights have been granted in writing by Taconic.
The Models and biological materials derived from them will not be distributed to third parties or used for commercial purposes.
Non-profit purchasers may not use this Model and/or biological materials derived from it in sponsored research or contract research studies unless it is purchased at the for-profit price.
Adoptive T cell transfer colitis data in RAGN12 mice
Representative data for adoptive T cell transfer colitis in RAGN12 mice provided by Bolder BioPATH. Download the full data package, including protocol information, colon weight and colon histopathology.
Representative data for adoptive T cell transfer colitis in RAGN12 mice provided by an anonymous pharmaceutical company. Download the full data package.
(Left) Body weights of Rag2 knockout mice following administration of CD4+CD45RBhiCD25- T cells. Mean values are indicated and bars represent SEM; *P<0.05, **P<0.01, ***P<0.001; n=4-6.
(Right) Representative gross colon pathology and splenomegaly of Rag2 knockout mice 60 days following injection with 1 x 106 CD4+CD45RBhiCD25- T cells of saline (control).
