The Rag1 Knockout/Transgenic OT-I T Cell Receptor line is homozygous for a transgene that encodes a T cell receptor specific for chicken ovalbumin 257-264, presented by the MHC class I molecule H-2Kb. It is also deficient in the Rag1 gene (recombinase reactivating gene 1) and therefore does not develop mature T or B cells expressing endogenous receptors. Virtually all peripheral CD8+ cells express the transgene. These mice are useful as a source of homogeneous donor CD8+ T cells for in vivo adoptive transfer studies to investigate T cell development, activation, memory, and tolerance. They can be used in conjunction with CD4+ T cells from the Rag2 Knockout/Transgenic OT-II T Cell Receptor line, which are specific for chicken ovalbumin 323-339, presented by the MHC class II molecule H-2 I-Ab, as a pair of helper and CTL T cells reactive against the same antigen.
Genetic Background: C57BL/6 Background
Origin: The OT-I microinjected model was developed by Dr. Francis Carbone of Monash Medical School and Dr. Michael Bevan of the University of Washington. The model was generated by injecting the rearranged T cell receptor Vα2-Jα26 (under the control of the alpha chain promoter and enhancer) and Vβ5-Dβ2-Jβ2.6 (under the control of the MHC class I promoter and the Ig heavy chain enhancer) into B6 x B6.C-H2bm1 blastocysts. Founders were subsequently bred to C57BL/6J mice for 9 generations. The line was later transferred to NIAID from Dr. Kristin Hogquist of the University of Minnesota and rederived by mating OT-I source males to C57BL/6NAi females. The transgenic mice were crossed twice to a homozygous C57BL/6J-Rag1tm1Mom knockout mouse which had been backcrossed 10 generations to B6/J (Mombaerts P, Cell 1992;68:869-877), and Rag1-/- offspring were selected. These mice were then intercrossed and OT-I TCR positive mice homozygous for the transgene were identified by test mating them to wild type partners and selecting animals that only gave rise to transgene positive offspring.
Availability: This model is unavailable.
Hogquist KA, Jameson SC, Heath WR, Howard JL, Bevan MJ and Carbone FR: T Cell receptor antagonist peptides induce positive selection
; Cell 1994: 76:17-27
Li M, Davey GM, Sutherland RM, Kurts C, Lew AM, Hirst C, Carbone FR, Heath WR.; Cell-associated ovalbumin is cross-presented much more efficiently than soluble ovalbumin in vivo
. J Immunol 2001 166 :6099-6103
Mombaerts P, Iacomini J, Johnson RS, Herrup K, Tonegawa S and Papaioannou VF: RAG-I deficient mice have no mature B and T cells
. Cell 1992 68(5):869