The Jh mouse was developed by Dennis Huszar et al. at GenPharm International. The model was created by targeting the Jh gene in AB01 embryonic stem cells derived from 129/SvEv mice and injecting the targeted cells into C57BL/6J blastocysts. Heterozygotes on a C57BL/6J background were intercrossed to generate homozygous targeted mutation mice. Yale University received stock from GenPharm in 1992. The mice were then backcrossed several generations to a C.B-Igh-1 b congenic background. The mice were then backcrossed two generations (N2) to a BALB/c inbred background and intercrossed to homozygosity. The colony is maintained through homozygous matings.
Chen J, Trounstine M, Alt FW, Young F, Kurahara C, Loring JF, Huszar D. (1993) Immunoglobulin Gene Rearrangement in B Cell Deficient Mice Generated by Targeted Deletion of the JH Locus. Int Immunol,5(6): 647-656.
Conditions of Use for Taconic Transgenic Models™
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