The Abb/B2m multiple targeted mutation model was generated through the mating of B2m and Abb lines from the Jaenisch laboratory at the Whitehead Institute and the Glimcher lab at Harvard University, respectively. Abb mice at three backcrosses (N3) onto C57BL/6 were mated to B2m at eight backcrosses (N8) onto C57BL/6 in 1992 by Laurie Glimcher. The mice were then backcrossed to N17. Taconic received stock through the NIAID repository and embryo transfer derived the line in 1996. The line is maintained through mating of double homozygotes.
Wild type for Nnt mutation
Grusby MJ, Auchincloss H Jr, Lee R, Johnson RS, Spencer JP, Zijlstra M, Jaenisch R, Papaioannou VE, Glimcher LH. (1993) Mice lacking major histocompatibility complex class I and class II molecules. Proc Natl Acad Sci USA, 90(9):3913-3917.