Figure 1: Experimental design used to generate CD1 mice with four different gut microbiota (GM) profiles. Schematic diagram showing embryo transfer scheme used to rederive CD1 mice to C57BL/6J GMJAX, C67BL/6NTacGMTAC, Crl:CD1GMCRL, and HSD:ICRGMHSD surrogate dams. At maturity, offspring were mated using an outbred mating scheme within each GM profile and maintained as four separate breeding colonies for nine generations.An increased interest in studies involving the microbiota requires animal models that appropriately mimic the extremely complex normal gut microbiota. Differences in rodent models can have a negative impact on a study's reproducibility.
On July 4, 2018, researchers at the University of Missouri, University of California, Davis, and University of North Carolina at Chapel Hill published a paper titled, "Development of outbred CD1 mouse colonies with distinct standardized gut microbiota profiles for use in complex microbiota targeted studies," in the journal Nature. This paper provides a methodology for transferring and maintaining complex gut microbiota in a CD1 mouse colony, which will enable researchers to improve the study reproducibility.