-
The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
-
Exon 2 contains the translation initiation codon.
-
Exons 4 to 8 have been flanked by loxP sites (size of loxP-flanked region: 3.2 kb).
-
The constitutive KO allele has been generated after Cre-mediated recombination by crossing chimeras to a Cre-Deleter on a C57BL/6 background.
-
The constitutive KO line has been derived using C57BL/6NTac animals and the Cre-transgene was removed by segregation.
-
Deletion of exons 4 to 8 should result in loss of function of the Scap gene by deleting the transmembrane domains TM2 and TM3 and part of the SSD domain and by generating a frameshift from exon 3 to exons 9 and 10 (premature Stop codon in exon 9).
-
The remaining recombination sites are located in non-conserved regions of the genome.
-
According to NCBI and Ensembl databases, the Ptpn23 gene (Entrez ID: 104831; ENSMUSG00000036057) is located approx. 10 kb downstream of Scap exon 8 in a tail-to-tail orientation.
-
The corresponding conditional KO allele is available as line 10938.
-
Datamining and Design performed in 2010.
Gene Family:
Chaperone Genetic Background:
C57BL/6 Gene Symbol:
Scap Gene Accession Number:
NM_001001144.1 Allele Type:
KO Source:
TaconicArtemis Availability:
Cryopreserved at Taconic
Species:
Mouse Official Gene Name:
SREBF chaperone
