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The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
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Exon 2 contains the translation initiation codon.
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Exons 4 to 8 have been flanked by loxP sites (size of loxP-flanked region: 3.2 kb).
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The constitutive KO allele has been generated after Cre-mediated recombination by crossing chimeras to a Cre-Deleter on a C57BL/6 background.
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The constitutive KO line has been derived using C57BL/6NTac animals and the Cre-transgene was removed by segregation.
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Deletion of exons 4 to 8 should result in loss of function of the Scap gene by deleting the transmembrane domains TM2 and TM3 and part of the SSD domain and by generating a frameshift from exon 3 to exons 9 and 10 (premature Stop codon in exon 9).
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The remaining recombination sites are located in non-conserved regions of the genome.
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According to NCBI and Ensembl databases, the Ptpn23 gene (Entrez ID: 104831; ENSMUSG00000036057) is located approx. 10 kb downstream of Scap exon 8 in a tail-to-tail orientation.
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The corresponding conditional KO allele is available as line 10938.
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Datamining and Design performed in 2010.
Gene Family:
Chaperone Genetic Background:
C57BL/6 Gene Symbol:
Scap Gene Accession Number:
NM_001001144.1 Allele Type:
KO Source:
TaconicArtemis Availability:
Cryopreserved at Taconic
Species:
Mouse Official Gene Name:
SREBF chaperone