Rab29 Overexpression Mouse

Targeted Transgenic

Rab29 Overexpression Mouse

C57BL/6NTac Mouse Background

  • Model #
  • Genotype
  • Nomenclature
  • 16552-F
    ki/wt
    C57BL/6NTac-Gt(ROSA)26Sortm1(Pgk-Rab29)Tac
  • 16552-M
    ki/wt
    C57BL/6NTac-Gt(ROSA)26Sortm1(Pgk-Rab29)Tac
  • Model 16552 is a constitutive knock-in of murine Rab29 under control of the Pgk promoter generated by targeted transgenesis into the ROSA26 locus.
  • Rab29 is a Rab GTPase that acts as a key regulator in vesicle trafficking and maintaining the endosome-trans-Golgi network structure. Rab29, together with LRRK2, plays a role in the retrograde trafficking pathway for recycling proteins.
  • Rab29 is one of five genes located within the PARK 16 locus that has been linked to Parkinson's disease.
  • Rab 29 is a direct substrate of LRRK2--a kinase protein in which autosomal dominant missense mutations are linked to Parkinson's disease through increased kinase activity. In addition, Rab29 is a regulator of LRRK2, controlling its localization and activation. Homozygous mice can be produced by breeding heterozygous females to heterozygous males, but production is very low. Taconic recommends breeding wild type C57BL/6NTac female by heterozygous male or heterozygous female by wild type C57BL/6NTac male.
  • There is a substantial increase in Rab29 protein levels by western blot in brain, kidney, and distal colon, with a moderate increase in spleen, lungs, macrophages. See "Phenotypic Data" section below for more detail.
Orders by weight: Taconic cannot accept orders by weight for this model. Please note that shipments may contain animals with a larger weight variation.

Ordering Note for Controls: Model 16552 has been generated and maintained on the C57BL/6NTac genetic background. A recommended wild type control option is to utilize C57BL/6NTac commercial animals sourced from our Cambridge City, Indiana production site (the same location where model 16552 is bred). Wild type littermate controls are also available upon request.

Genetic Background:

C57BL/6NTac Background

Origin:

The Rab29 Mouse was generated by inserting a transgene containing a neo cassette, a Pgk promoter, the murine Rab29 open reading frame together with a Kozak sequence (GCCACC), the human growth hormone (hGH) polyadenylation signal and an additional polyadenylation signal into the ROSA26 via recombinase-mediated cassette exchange (RCME). The original purpose of the Neo cassette was to allow for positive selection for construct recombination in the ES cell stage. Once in place, the presence of the polyA sequence following the Neo construct prevents ‘read-through’ transcription from the endogenous ROSA26 locus. This allows for constitutive expression of Rab29 under control of the Pgk promoter. Targeting occurred in ES cells derived from C57BL/6NTac. Targeted ES cells were microinjected into BALB/c blastocysts to generate chimeras. Germline transmission occurred during an IVF expansion using chimeric males and C57BL/6NTac oocyte donors. The colony is maintained by mating wild type C57BL/6NTac females to heterozygous males and heterozygous females to wild type C57BL/6NTac males.

Availability: This colony is still under development and only limited quantities are currently available.

As of April 1, 2018, The Michael J. Fox Foundation for Parkinson's Research (MJFF) has instituted a new ordering policy for this model in an effort to prevent delays in order fulfillment, ensure models are shipped at the desired age, and maintain affordable pricing for all researchers accessing MJFF models. To achieve these goals, the new MJFF policy dictates that no one group can deplete the MJFF-sponsored colony. As such, orders will be limited to a total quantity of approximately 15-20 per researcher per month. Average lead time and age range may vary based on quantity and sex requested. Researchers requiring orders above this amount should establish a dedicated colony for their own use, either by breeding in-house or contracting with Taconic. For questions or concerns, please email tools@michaeljfox.org.

Source:

Model Sponsor: The Michael J. Fox Foundation

Color:

Black

Species:

Mouse

Initial Publication:

This model is not yet published.


Label License Agreement and Conditions of Use for Rab29 Overexpression Mouse #16552
The Rab29 Overexpression Mouse ("Model") is produced and distributed under rights to patents and/or intellectual property owned by or licensed to the Michael J. Fox Foundation ("MJFF") and Taconic. Taconic has rights to breed and distribute the Model. MJFF permits the sale of such Model to each purchaser that agrees to the Terms and Conditions of the Sale and following terms of use:
  • Title to the Model, any progeny and biological materials derived from the foregoing remains with MJFF.
  • The Model may be bred or crossbred by purchaser or by Taconic for purchaser.
  • Purchaser shall inform MJFF of any crossbred animal generated ("Modified Progeny"), shall at MJFF's election deposit breeding pairs of each Modified Progeny with Taconic or another designee of MJFF and shall permit Taconic or another designee of MJFF to distribute the Modified Progeny on behalf of MJFF to non-profit institutions or for-profit institutions without any compensation to purchaser on terms similar to the terms and conditions by which purchaser received the Model. Purchaser assumes all liability for any claim or demand that Modified Progeny created by purchaser infringes on the intellectual property rights of any third party.
  • The Model and progeny may be used by non-profit institutions or for-profit institutions for research purposes only.
  • The Model, progeny or biological materials will not be used for commercial purposes.
  • The Model, progeny, and biological materials derived from them will not be distributed to any third party.
  • Purchaser assumes all risk from using the Model, progeny and biological materials.
  • To the extent permissible by law, purchaser indemnifies, defends, and holds harmless MJFF from any claim or demand that purchaser's use or transfer of the Model, progeny, or biological materials has caused harm, damage or liability.
Label License Agreement and Conditions of Use - Printable Version

Analysis of Rab29 mRNA levels in Rab29 Overexpression Mouse

Analysis of Rab29 mRNA levels in Rab29 Overexpression Mouse: Brain Analysis of Rab29 mRNA levels in Rab29 Overexpression Mouse: Kidney Analysis of Rab29 mRNA levels in Rab29 Overexpression Mouse: Lungs
Four biological replicates per genotype and two technical replicates per reaction. Data courtesy of Dr. Dario Alessi.

Analysis of Rab29 protein levels in Rab29 Overexpression Mouse

Analysis of Rab29 protein levels in Rab29 Overexpression Mouse
Data courtesy of Dr. Dario Alessi.