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This double targeted mutation contains a disruption of both Rag2 and Pfp genes
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Exhibits a severe depletion of NK cell function through the disruption of the Pfp gene and lacks mature T or B lymphocytes through disruption of the Rag2 gene
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Used to study overall regulation of the immune system or specific areas such as NK or CTL activity, immune suppression and transplantation
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Carries the H2b haplotype
Genetic Background:
C57BL/6 Background Origin:
The Pfp/Rag2 mouse was developed by crossbreeding the Pfp targeted mutation mouse (model PFPN12) and the Rag2 targeted mutation mouse (model RAGN12) at Taconic and breeding to homozygosity for both genes. This model was backcrossed twelve generations (N12) to C57BL/6NTac. The colony was maintained through homozygous matings. Genetics:
Wild type for Nnt mutation Color:
Black Species:
Mouse Initial Publication:
Shinkai, Y, Rathbun, G, Lam, KP, Oltz, EM, Stewart, V, Mendelsohn, M, Charron, J, Datta, M, Young, F, Stall, AM, and Alt, FW. (1992)
RAG-2-Deficient Mice Lack Mature Lymphocytes Owing to Inability to Initiate V(D)J Rearrangement,
Cell, 68: 855-867.
Walsh CM, Matloubian M, Liu C, Ueda R, Kurahara CG, Christensen JL, Huang MTF, Young JD, Ahmed R, and Clark WR. (1994)
Immune function in mice lacking the perforin gene.
Proc Natl Acad Sci USA, 91(23): 10854-10858.
