The transgenic construct contains 5´ the Mouse Mammary Tumor virus (MMTV) promoter, Ki-Ras element, rabbit b-globulin intron, human NUMB cDNA, and SV40 polyA signal at 3´ end.
The linearized transgenic construct was microinjected in C57BL/6 mice by pronuclear injection.
Putative transgenic founders were identified by genotyping and bred for germline transmission with C57BL/6 mice.
Heterozygous transgenic mice were identified by genotyping.
Only low expression levels of the human transgene is detected in salivary gland, mammary gland, and lung.
Datamining and Design performed in 2006.
Gene Family: Phosphoprotein
Genetic Background: C57BL/6
Gene Symbol: Numb
Gene Accession Number: NM_001136075
Allele Type: TG
Source: Institut Clinique de la Souris (ICS), Illkirch, France
Availability: Cryopreserved at Taconic
Official Gene Name: numb gene homolog (Drosophila)