The targeting vector has been generated using 129 DNA and transfected into 129 ES cell line.
Klrd1 exon 3 has been disrupted and exon 4 removed by the neomycin resistance cassette, which is flanked by loxP sites.
The neomycin resistance gene has been deleted by transient transfection of Cre in targeted ES cells.
Chimeric mice were produced and germ line transmission achieved.
The constitutive KO line has been subsequently backcrossed to C57BL/6 animals for 9 generations.
Deletion of parts of exon 3 and entire exon 4 should result in loss of function of the Klrd1 gene by deleting a dimerization and the C-type lectin-like domains.
The remaining recombination sites are located in intron regions of the genome.
See M.T. Orr et al, PLoS ONE 5, e15184, 2010, for further details.
Gene Family: Receptor
Genetic Background: C57BL/6;129SvJ
Gene Symbol: Klrd1
Gene Accession Number: NM_010654
Allele Type: KO
Availability: Cryopreserved at Taconic
Official Gene Name: killer cell lectin-like receptor, subfamily D, member 1