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The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
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Exon 1 contains the translation initiation codon.
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Exons 4 to 8 have been flanked by loxP sites (size of loxP-flanked region: approx. 3 kb).
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The constitutive KO allele has been generated after Cre-mediated recombination by crossing chimeras to a Cre-Deleter on a C57BL/6 background.
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The constitutive KO line has been derived using C57BL/6NTac animals and the Cre-transgene was removed by segregation.
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Deletion of exons 4 to 8 should result in loss of function of the Gckr gene by deleting the region containing the amino acids 96-215 and by generating a frameshift from exon 3 to exon 9.
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According to NCBI and Ensembl databases, the Fndc4 gene (NCBI ID: 64339, Ensembl: ENSMUSG00000038552) is located approx. 3 kb upstream of Gckr exon 4 in a head-to-head orientation.
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The corresponding conditional KO allele is available as line 10063.
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Datamining and Design performed in 2008.
Gene Family:
Isomerase Genetic Background:
C57BL/6 Gene Symbol:
Gckr Gene Accession Number:
NM_144909.1 Allele Type:
KO Source:
TaconicArtemis Availability:
Cryopreserved at Taconic Species:
Mouse Official Gene Name:
glucokinase regulatory protein
