The targeting vector has been generated using C57BL/6J DNA and transfected into Taconic's C57BL/6N Tac ES cell line.
Clec4a2 exon 1 contains the translation initiation codon.
Exons 1 to 3 have been replaced by the FRT site flanked neomycin resistance gene cassette.
The neomycin resistance gene cassette has been removed by transient transfection of Flp recombinase into targeted ES cell clones.
The constitutive KO line has been derived using C57BL/6NTac animals.
Deletion of exons 1 to 3 should result in loss of function of the Clec4a2 gene.
The remaining recombination sites are located in non-conserved regions of the genome.
Datamining and Design performed in 2005.
Gene Family: Receptor
Genetic Background: C57BL/6
Gene Symbol: Clec4a2
Gene Accession Number: NM_001170332
Allele Type: KO
Availability: Cryopreserved at Taconic
Official Gene Name: C-type lectin domain family 4, member a2