β-actin-luc - Model 10500

Random Transgenic

β-actin-luc (Model 10500) Random Transgenic Mouse Model
EZcohort™ Models
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This model has been replaced by an improved model - β-actin-luc - model 11977

Mixed Background

  • Model #
  • Genotype
  • Nomenclature
  • 10500-F
    tg/wt
    B6;FVB-Tyrc-2J Tg(Actb-luc)46Xen
  • 10500-M
    tg/wt
    B6;FVB-Tyrc-2J Tg(Actb-luc)46Xen
  • Carries a 14 kb fragment of the murine β-actin promoter isolated from genomic DNA, a chimeric intron and modified firefly luciferase cDNA (Promega pGL3)
  • Basal expression of the reporter is highest in skeletal muscle, thymus, skin, heart, bone, pancreas, and is detectable in all tissues, including white blood cells
  • The reporter is constitutively expressed and is not significantly inducible
  • Useful as a donor animal for studying the transplantation of various tissue types
  • SNP testing has shown that the strain background is equivalent only to 4-5 backcrosses onto B6 albino, so the background is designated as B6;FVB. Taconic will further backcross onto B6 albino and offer an improved model in the future.

Genetic Background:

B6;FVB Background

Origin:

The β-actin-luc mouse was developed by Caliper Life Sciences. The model was created by microinjecting a transgene containing a fragment of the murine β-actin promoter isolated from genomic DNA, a chimeric intron and modified firefly luciferase cDNA from pGL3. This transgene was microinjected into FVB/N zygotes. The resultant mice from founder line 46 were bred to FVB/NTac mice. The line was backcrossed the equivalent of 4-5 times onto the C57BL/6J-Tyrc-2J and intracrossed to make the coat color mutation homozygous. Taconic received stock from Caliper in 2010, and the line was embryo transfer derived. In the production colony, the line is maintained by mating mice which are wild type to those which are hemizygous for the luciferase transgene.


Availability:

This model has been replaced by an improved model - β-actin-luc - model 11977

Color:

Albino

Species:

Mouse

Initial Publication:

There is no specific publication describing the generation of these mice, but multiple publications exist demonstrating applications using the mice. See reference list.

 

Other publications:

Cheeran MC, Mutnal MB, Hu S, Armien A, Lokensgard JR. (2009) Reduced lymphocyte infiltration during cytomegalovirus brain infection of interleukin-10-deficient mice. J Neurovirol. 15(4):334-42.

Murphy CT, Moloney G, Macsharry J, Haynes A, Faivre E, Quinlan A, McLean PG, Lee K, O'Mahony L, Shanahan F, Melgar S, Nally K. (2010) Technical Advance: Function and efficacy of an {alpha}4-integrin antagonist using bioluminescence imaging to detect leukocyte trafficking in murine experimental colitis. J Leukoc Biol. 88(6):1271-8.

Murphy CT, Moloney G, Hall LJ, Quinlan A, Faivre E, Casey P, Shanahan F, Melgar S, Nally K. (2010) Use of bioluminescence imaging to track neutrophil migration and its inhibition in experimental colitis. Clin Exp Immunol. 162(1):188-96.

Schachtele SJ, Hu S, Little MR, Lokensgard JR. (2010) Herpes simplex virus induces neural oxidative damage via microglial cell Toll-like receptor-2. J Neuroinflammation. 7:35.

 



Conditions of Use for EZcohort™ Models
EZcohort™ models are produced and distributed under rights to patents and intellectual property licensed from various institutions. Taconic Biosciences, Inc. (Taconic) sells EZcohort™ models to purchasers, grants to each purchaser a right under Taconic's rights in such licensed patents and intellectual property to use the purchased EZcohort™ models in consideration of purchasers' acknowledgement of and agreement to Taconic's Terms and Conditions for the Sale of Products and the following conditions of use:

  • Title to EZcohort™ models and biological materials derived from them remains with Taconic.
  • EZcohort™ models will be used for research purposes only.
  • EZcohort™ models and biological materials derived from them will not be distributed to third parties or used for commercial purposes.
  • Breeders from deliverables can be used for propagation of EZcohort™ models or to cross to other strains.
  • Continuation of breeding and crossbreeding of EZcohort™ models outside Taconicis permitted for a term of three years post-purchase of the EZcohort™ models and is subject to annual confirmation of these EZcohort™ Conditions of Use.

Continuation of breeding and crossbreeding after the three year term will require renewal of the EZcohort™ Conditions of Use and payment of a fee.
Luciferase Expression of Transgenes in β-Actin-luc Mice
Figure 1A: Male and female mice were imaged in the ventral (left) and dorsal (right) regions. High basal luciferase expression was observed in males and females in feet, tail, ear, and mouth, all areas where bare skin is visible. Luciferase expression was observed in male gonads in some animals.

Figure 1B. β-actin-luc tissue expression survey from 2 male and 2 female mice (Fig. 1A). Data are expressed in Relative Light Units (RLU)/mg protein.
Luciferase Expression in Tissue Extracts

Tissue Expression of Luciferase signal

Luciferase expression of the β-actin-luc transgene was examined in extracts of tissue dissected from adult male and female mice from the β-actin-luc LPTA® animal model. Luciferase expression was highest in skeletal muscle, thymus skin, heart, bone, pancreas, and was detectable in all tissues examined including white blood cells (Fig. 1B). The bar graph is plotted with a logarithmic ordinate scale to graphically display all data. The expression data agree with ex vivo study data (not shown) and published work examining constitutive promoter-driven transgene expression.1,2 Luciferase expression was also examined in white blood cells from β-actin-luc mice (Fig. 1C (below)). Whole blood was harvested and spun to enrich for white blood cells. Cells were then serially diluted in a 96-well plate and placed in media containing luciferin substrate.

Luciferase Expression in β-Actin-Luc Circulating White Blood Cells
Figure 1C: Luciferase Expression in β-Actin-Luc Circulating White Blood Cells

Imaging Recommendations

Anesthetize mice prior to injection of luciferin and measurement of luciferase transgene expression. Optimal imaging occurs between 10 and 20 minutes following intraperitoneal injection of luciferin.