-
The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
-
Exon 1 contains the translation initiation codon.
-
Exons 1 to 2 and approximately 1.6 kb of sequence upstream of exon 1 (promoter region) have been flanked by loxP sites (size of loxP-flanked region: 3.7 kb).
-
The constitutive KO allele has been generated after Cre-mediated recombination by crossing chimeras to a Cre-Deleter on a C57BL/6 background.
-
The constitutive KO line has been derived using C57BL/6NTac animals and the Cre-transgene was removed by segregation.
-
Deletion of exons 1-2 (including part of the proximal promoter and 3´ UTR) should result in loss of function of the Amac1 gene by deleting the complete gene.
-
The remaining recombination sites are located in non-conserved regions of the genome.
-
According to NCBI and Ensembl databases, the Polr2a gene (Entrez ID: 20020; ENSMUSG0000005198) is located approx. 1.7 kb downstream of Amac1 exon 2 in a tail-to-head orientation.
-
The corresponding conditional KO allele is available as line 10437.
-
Datamining and Design performed in 2009.
Gene Family:
Dehydrogenase Genetic Background:
C57BL/6 Gene Symbol:
Amac1 Gene Accession Number:
NM_019871.2 Allele Type:
KO Source:
TaconicArtemis Availability:
Cryopreserved at Taconic
Species:
Mouse Official Gene Name:
acyl-malonyl condensing enzyme 1
