- The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
- Exon 1 contains the translation initiation codon.
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Exons 2 to 6 have been flanked by loxP sites (size of loxP-flanked region: 2.6 kb).
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The constitutive KO allele has been generated after Cre-mediated recombination by crossing chimeras to a Cre-Deleter on a C57BL/6 background.
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The constitutive KO line has been derived using C57BL/6NTac animals and the Cre-transgene was removed by segregation.
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Deletion of exons 2 to 6 should result in loss of function of the Acsf2 gene by deleting part of the catalytic domain and by generating a frameshift from exon 1 to exons 7 and 8 (premature Stop codon in exon 7).
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The remaining recombination sites are located in non-conserved regions of the genome.
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According to NCBI and Ensembl databases, there are 2 genes in the neighborhood of the Acsf2 gene:
- The Chad gene (Entrez ID:12643, ENSMUSG00000039084) is located into Acsf2 intron 10 (182 bp downstream of Acsf2 exon 10) in tail-to-tail orientation.
- The Rsad1 gene (Entrez ID:237926, ENSMUSG00000039096) is located 22 kb downstream of Acsf2 exon 6 in a head-to-tail orientation.
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The corresponding conditional KO allele is available as line 11199.
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Datamining and Design performed in 2009.
Gene Family:
Synthetase Genetic Background:
C57BL/6 Gene Symbol:
Acsf2 Gene Accession Number:
NM_153807.2 Allele Type:
KO Source:
TaconicArtemis Availability:
Cryopreserved at Taconic
Species:
Mouse Official Gene Name:
acyl-CoA synthetase family member 2
