Cardiovascular Disease: Soluble ICAM
Recruitment of activated phagocytes occurs as part of the inflammatory response and is mediated by cell-adhesion molecules that are induced on the surface of local blood vessel endothelium. Lipopolysaccharide (LPS) initiates the inflammatory response in mice by mounting an acute phase response. This response is induced by the production of TNFα, and other cytokines from activated monocytes and neutrophils. The activation of endothelium is driven in particular by TNFα, and the inflamed endothelium expresses two adhesion molecules, ICAM-1 and ICAM-2. These intercellular adhesion molecules mediate leukocyte-endothelium adhesion. Studies in knockout mice have demonstrated the relationship between sICAM expression and atherosclerosis progression. For example, the level of sICAM-1 increases significantly in parallel with the progression of atherosclerosis in APOE-deficient mice, while the sICAM-1 level remains constant in wild type mice (Kitagawa K et al., 2002). The mouse sICAM-1 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse sICAM-1 levels in serum and plasma. The sample utilized for testing is acquired from the animals 3 hours following injection with a sub-lethal dose of LPS. Intracellular adhesion molecule-1 is an inducible transmembrane molecule that plays a role in cell migration, antigen presentation, and leukocyte activation.
Displayed below is a sample graph of how soluble ICAM-1 observations are presented. In comprehensive phenotypic data packages graphs are interactive. Raw or calculated data and statistics can be seen by clicking on points in the graph.
Figure illustrates sICAM-1 levels in serum from male and female mutant mice. sICAM-1 values of wild type littermates (green circle), homozygous (red diamond), and recent historical wild type (purple line) mice are plotted against long-term historical values (± 2 standard deviations) for wild type animals (green shading). Recent wild type values are calculated from data collected within 60 days of current measures and long-term historical values are derived from data collected on more than 10,000 wild type mice.
Kitagawa K, Matsumoto M, Sasaki T, Hashimoto H, Kuwabara K, Ohtsuki T, Hori M. (2002) Involvement of ICAM-1 in the progression of atherosclerosis in APOE knockout mice, Atherosclerosis, 60(2):305-10.