The cannulated IOP measurement test measures the baseline pressure inside the mouse's eye. Pressure is measured by placing a saline-filled glass microneedle through the mousse cornea and into the anterior chamber. The pressure in the eye is transmitted through the saline filled needle, which is attached to a pressure transducer. The pressure transducer then relays the pressure reading through an amplifier to a computer via a data acquisition system where it is then recorded. Mice are anesthetized with Avertin. IOP is monitored for 90 seconds.
Displayed below is a sample graph of how intraocular pressure measurements are presented. In comprehensive phenotypic data packages graphs are interactive. Raw or calculated data and statistics can be seen by clicking on points in the graph.
Figure illustrates intraocular pressure (IOP) measures of male (left) and female (right) mutant mice. IOP values of wild type littermates (green circle), homozygous (red diamond), and recent historical wild type (purple line) mice are plotted against long-term historical values (± 2 standard deviations) for wild type animals (green shading). Recent wild type values are calculated from data collected within 60 days of current measures and long-term historical values are derived from data collected on more than 10,000 wild type mice.