The Gfap-luc mouse was developed by Caliper Life Sciences. The model was created by microinjecting a transgene containing a fragment of the glial fibrillary acidic protein promoter, a human β-globin intron 2 and modified firefly luciferase cDNA from pGL3. This transgene was microinjected into FVB/N zygotes. The resultant mice from founder line 53 were bred to FVB/NTac mice and intracrossed to homozygosity. Taconic received stock from Caliper in 2010. The line was embryo transfer derived. In the production colony, the line is maintained by mating homozygous male and female mice.
Genetics: Initial Publication: Other References:
Animal Diet: NIH #31M Rodent Diet Licensing Requirements: LPTA® Conditions of Use
Zhu L, Ramboz S, Hewitt D, Boring L, Grass DS, Purchio AF. (2004) Non-invasive imaging of GFAP expression after neuronal damage in mice. Neurosci Lett. 367(2):210-2.
Luo J, Ho PP, Buckwalter MS, Hsu T, Lee LY, Zhang H, Kim DK, Kim SJ, Gambhir SS, Steinman L, Wyss-Coray T. (2007) Glia-dependent TGF-beta signaling, acting independently of the TH17 pathway, is critical for initiation of murine autoimmune encephalomyelitis. J Clin Invest. 117(11):3306-15.
Luo J, Ho P, Steinman L, Wyss-Coray T. (2008) Bioluminescence in vivo imaging of autoimmune encephalomyelitis predicts disease. J Neuroinflammation. 5:6.
Cordeau P Jr, Lalancette-Hébert M, Weng YC, Kriz J. (2008) Live imaging of neuroinflammation reveals sex and estrogen effects on astrocyte response to ischemic injury. Stroke. 39(3):935-42.
Keller AF, Gravel M, Kriz J.(2009) Live imaging of amyotrophic lateral sclerosis pathogenesis: disease onset is characterized by marked induction of GFAP in Schwann cells. Glia. 57(10):1130-42.
Tamgüney G, Francis KP, Giles K, Lemus A, DeArmond SJ, Prusiner SB. (2009) Measuring prions by bioluminescence imaging. Proc Natl Acad Sci USA. 106(35):15002-6.
Lee J, Borboa AK, Baird A, Eliceiri BP. (2011) Non-invasive quantification of brain tumor-induced astrogliosis. BMC Neurosci. 12:9.
Watts JC, Giles K, Grillo SK, Lemus A, DeArmond SJ, Prusiner SB. (2011) Bioluminescence imaging of Abeta deposition in bigenic mouse models of Alzheimer's disease. Proc Natl Acad Sci USA. 108(6):2528-33.
Please inquire for timeline and pricing for F1 hybrids of the Gfap-luc and B6 mice for use in EAE studies
Animal Diet: NIH #31M Rodent Diet
Licensing Requirements: LPTA® Conditions of Use
Kainic acid treatment study:
Figure 1: The Gfap-luc transgene was induced following kainic acid treatment. Age-matched adult mice were treated for four days. Treatment with kainic acid triggered higher luciferase expression in brain, with increased expression and neuronal damage to the hippocampus (data not shown).
Meningitis infection study: The Gfap-luc transgene was induced to express luciferase in the brain (Figure 2) and the lumbar spine (Figure 3) of mice infected with Streptococcus pneumonia.
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