Taconic Transgenic Exchange

Ahr Knockout Mouse

tADMET™ Model

Licensing Requirements

Model Number	Zygosity	Nomenclature
9166-F	Homozygous Deletion	C57BL/6-Ahr^tm1.2Arte
9166-M	Homozygous Deletion	C57BL/6-Ahr^tm1.2Arte

Model Description

This targeted mutation strain carries a deletion of the mouse aryl hydrocarbon recpetor (Ahr) gene.
Useful in prediction of CYP induction and therefore pharmacokinetics, drug toxicity and efficacy in humans.
Mice which are homozygous knockouts for the Ahr gene are smaller than heterozygotes or wild types. As described in the literature, dead newborn and missing litters are common in Ahr knockout animals, with some incidence of death after weaning also seen. Taconic recommends that this line be ordered no earlier than 6 weeks of age, and 8-10 weeks of age or older may be preferable, particularly if transit times will be longer than 2 days.

Origin: The Ahr Knockout mouse was developed by Taconic in collaboration with CXR Biosciences. It was generated by crossing an AHR humanized mouse line (Model # 9165) with a Cre deleter mouse. The Humanized AHR Mouse model was created through a targeted replacement of mouse Ahr Exon 3 with a cDNA construct containing human AHR Exons 3-11 in C57BL/6NTac-derived ES cells. Targeted ES cells were injected into BALB/cJBomTac blastocysts and the resultant chimeras were backcrossed to a Flpe deleter strain on C57BL/6J to eliminate selection markers. The Ahr Knockout Mouse model was derived from the Humanized AHR Mouse line through a Cre-mediated deletion of the human AHR sequence. Mouse exon 3 is deleted in the knockout, resulting in an out of frame splicing of exons 2 to exon 4. The absence of functional Ahr protein was proven experimentally. Taconic received stock in 2009, and the line was embryo transfer derived. The colony is maintained by mating homozygotes.

Color: Black

Genetics:

Initial Publication: Not published yet.

Animal Diet: NIH #31M Rodent Diet

Licensing Requirements:

Label License: Conditions of Use - Model 9166
This Model is produced and distributed under rights to patents and intellectual property owned by Taconic or licensed from various institutions. Taconic sells this model to purchaser, and grants to each purchaser a right under Taconic's own or sublicensable rights in such licensed patents and intellectual property to use the purchased Model in consideration of purchasers' acknowledgement of and agreement to the Terms and Conditions of Sale and the following terms of use:

Title to this Model and biological materials derived from it remains with TACONIC FARMS, INC.
The Model will be used for research purposes only.
The Model will not be bred except to obtain embryos or fetuses required for research purposes
The Model and biological materials derived from it will not be distributed to third parties or used for commercial purposes
Non-profit purchasers may not use this Model and/or biological materials derived from it in sponsored research or contract research studies unless it is purchased at the for-profit price.

Pricing for non-profit users (excluding users at non-profit foundations which are affiliated with a for-profit entity):