Taconic's innovative RNAi models enable you to reversibly induce RNA mediated gene knockdown, in vivo, at selected time points. In the absence of inducer (doxycycline), the tet-repressor protein binds to the tet-operator control element and effectively inhibits shRNA expression from the H1 promoter. When doxycycline is administered through an animal's feed or drinking water, it binds to the tet-repressor protein and blocks its interaction with the H1 promoter. This is followed by H1 promoter activation, shRNA expression and effective RNA mediated gene-knockdown. Withdrawal of doxycycline inhibits shRNA expression resulting in pre-knockdown levels of target gene expression.
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