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Animal Models

Fpr1 Knockout

Emerging Model Knockout Mouse

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Model Sponsor: Institution-NIAID
 

Model Number Zygosity Nomenclature
4169-F Homozygous C57BL/6NTac-Fpr1tm1Gao N6
4169-M Homozygous C57BL/6NTac-Fpr1tm1Gao N6
  • Description
  • Price Table

Model Description


    N-formylpeptides derive from bacterial and mitochondrial proteins, and bind to specific receptors on mammalian phagocytes. Since binding induces chemotaxis and activation of phagocytes in vitro, it has been postulated that N-formylpeptide receptor signaling in vivo may be important in antibacterial host defense, although direct proof has been lacking. Mice genetically deficient in formyl peptide receptor (FPR-/-) have no obvious developmental defects and do not develop spontaneous infection when derived in specific pathogen-free conditions. This suggests that, under these conditions, FPR is dispensable. However, when challenged with Listeria monocytogenes, FPR-/- mice have accelerated mortality and increased bacterial burden in liver and spleen early after infection, which suggests a role for FPR in host defense, specifically through regulation of innate immunity.

Origin:
Drs. Ji-Liang Gao and Philip Murphy in the Laboratory of Molecular Immunology, NIAID, made the FPR-/- mouse by targeted gene disruption in 1999. The Fpr1 gene was mutated by replacing a 150 bp DNA fragment in the Fpr1 open reading frame with a neomycin resistance gene. The disrupted Fpr1 gene was introduced to 129/Sv ES cells to create chimeric mice, and then the chimeric founders were backcrossed to C57BL/6NAi mice for 10 generations in the NIAID barrier facility at Taconic. At this point, it was discovered that the C57BL/6NAi line had become contaminated with the IFNγ knockout gene. Line 4169 was then backcrossed to C57BL/6NTac (Taconic B6) for 5 generations in order to eliminate the contaminating gene and put the strain onto the Taconic C57BL/6 sub-line background.

Color: Black

Genetics:

Initial Publication:
Gao J-L, Lee E, and Murphy PM. (1999) Impaired anti-bacterial host defense in mice lacking the N-formylpeptide receptor. J. Exp. Med., 189: 657-662.

Animal Diet: NIH #31M Rodent Diet

Licensing Requirements:
Execution of NIAID Material Transfer Agreement

Price Table


4169 (in U.S. Dollars)

MaleFemale
$40.00$40.00
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