Conditional Targeted Transgenesis

Conditional Targeted Transgenesis is the extremely efficient introduction of a gene at a well-characterized transcriptionally active locus in the mouse genome. Expression of your transgene is repressed by a strong transcriptional termination site (stop cassette), flanked by LoxP sites, which is inserted 5' to your gene of interest In combination with the appropriate Cre-expressing mouse line, this is a versatile tool for tissue-specific or inducible expression of transgenes

Generation of Mouse Models with targeted transgene into the ROSA26 locus consists of the following major steps:

1. Targeting Vector Design & Construction
2. Targeted B6129S6F1 or C57BL/6 ES cells
3. Generation of mice heterozygous for the targeted transgene insertion

Expected timeline: 18-22 WEEKS for B6129S6F1 ES cells or 36 WEEKS for C57BL/6 ES cells

Taconic has incorporated rigorous quality control and documentation of different mouse production steps using project management software and the Laboratory Information Management System (LIMS). A designated Taconic scientific project manager will ensure optimal communication and strict adherence to the timeline and deliverables throughout the project. Taconic will provide a detailed Milestone Documentation after each of the steps mentioned above.

Upon generation of the line at Taconic, we will coordinate the delivery and further breeding of your animals to produce the research cohorts you want at the health status you need.