COX - 2

Model Description

• Contains a disruption of the Ptgs2 gene, one of the prostaglandin endoperoxide synthase/cyclooxygenase genes, preventing synthesis of cyclooxygenase-2 (COX-2) protein
• COX-1 and COX-2 are key cyclooxygenase isoforms that catalyze key steps in the prostaglandin synthesis
• Non-steroidal anti-inflammatory drugs (NSAIDs) target COX isoforms to inhibit them as a primary means of reducing inflammation
• Unlike COX-1, Cox-2 is normally undetectable in most tissues; inflammatory stimuli can induce macrophages and other migratory cells to synthesize it
• Homozygotes exhibit normal inflammatory response to bacterial assault and to arachidonic acid and other inflammatory agents
• Although survival of weaned animals is normal, homozygous mice develop peritoneal lesion and exhibit severe, progressive renal pathology and lesions of varying severity that cause a progressive deterioration with aging
• Useful to examine the redundant and compensatory responses of the cyclooxygenase gene, to study cyclooxygenase inhibitors and NSAIDs, to examine the role of COX enzymes in inflammatory disease and to study the role of COX enzymes in pregnancy and parturition

Origin: The COX-2 mouse was developed in the laboratories of Robert Langenbach at NIEHS and Oliver Smithies at University of North Carolina in 1995 . The model was created by targeting the Ptgs2 gene in E14TG2a embryonic stem cells derived from 129P2/OlaHsd mice and injecting the targeted cells into C57BL/6J blastocysts. Resultant chimeras were backcrossed to C57BL/6J mice. Taconic received stock in September 1998 for embryo transfer derivation into the NIEHS repository. The model was transferred from NIEHS to Taconic in 2002 and derived by embryo transfer. The line is maintained on a mixed B6;129P2 background by breeding homozygous males to heterozygous females. A separate wild type colony is maintained.

Color: Various

Genetics: Wildtype for Nnt mutation

Initial Publication: Morham SG. Langenbach R, Loftin CD, Tiano HF, Vouloumanos N, Jennette JC, Mahler JF, Kluckman KD, Ledford A, Lee CA, Smithies O. (1995) Prostaglandin Synthase 2 Gene Disruption Causes Severe Renal Pathology in the Mouse. Cell, 83(3):472-482.

Animal Diet: NIH #31M Rodent Diet

Licensing Requirements:

Label License: Conditions of Use for Taconic Transgenic Models™
Taconic Transgenic Models (Models) are produced and distributed under rights to patents and intellectual property licensed from various institutions. Taconic sells the Models to purchasers, and grants to each purchaser a right under Taconic's rights in such licensed patents and intellectual property to use the purchased Model in consideration of purchasers' acknowledgement of and agreement to the Terms and Conditions of Sale and the following terms of use:

• Title to these Models and biological materials derived from them remains with Taconic Farms, Inc
• The Models will be used for research purposes only
• The Models will not be bred except to obtain embryos or fetuses required for research purposes
• The Models and biological materials derived from them will not be distributed to third parties or used for commercial purposes.

Related Reports

View the production locations(s) and the accompanying current IHMS^TM Health Report and Taconic Health Standard for this model

Price Table

2181 (in U.S. Dollars)

2181 Heterozygous or Wild Type (in U.S. Dollars)

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