HRN™

Model Description

• HRN stands for Hepatic cytochrome P450 Reductase Null
• Mice are homozygous for both the floxed Por allele and the Alb-cre transgene
• Exhibits liver-specific conditional disruption of the Por gene; POR is the electron donating enzyme for all cytochrome P450 enzymes
• Lacks first pass metabolism by P450 enzymes in the liver
• Can be used to determine the role of hepatic P450 metabolism in drug disposition
• Useful for in vivo efficacy studies using smaller amounts of compound
• Can provide information on whether parent or metabolite compounds are responsible for observed efficacy or toxic effects
• Offered through a partnership with CXR Biosciences

Origin: The HRN mouse was developed in the laboratory of C. Roland Wolf of the Ninewells Hospital & Medical School. The model was created by targeting the Por gene to generate a floxed allele in GK129/1 embryonic stem cells derived from 129P2 mice and injecting the targeted cells into C57BL/6 blastocysts. Resultant chimeras were backcrossed to C57BL/6 for one generation. Mice heterozygous for the floxed Por allele were intercrossed to generate mice homozygous for the floxed Por allele on a mixed B6;129P2 background. The Alb-cre transgene was developed in the laboratory of Mark A. Magnuson at Vanderbilt University School of Medicine by microinjecting Cre recombinase gene under the control of the rat albumin enhancer/promoter into B6D2F2 zygotes. Mice homozygous for the floxed Por allele were bred to carriers for the Alb-cre transgene to generate HRN mice. The HRN model was backcrossed to C57BL/6J a total of 6 generations (N6). Taconic received stock from CXR Biosciences in April 2007. The mice were backcrossed to C57BL/6NTac (N7) and embryo transfer derived. The colony is maintained through mating of mice that are homozygous for the floxed Por allele and homozygous for the Alb-cre transgene.

Color: Black

Initial Publication: Henderson CJ, Otto DME, Carrie D, Magnuson MA, McLaren AW, Rosewell I, Wolf CR. (2003) Inactivation of the hepatic cytochrome P450 system by conditional deletion of hepatic cytochrome P450 reductase. J Biol Chem 278(15):13480-13486.

Animal Diet: NIH #31M Rodent Diet

Licensing Requirements:
Label License: Conditions of Use for Taconic Transgenic Models™
Taconic Transgenic Models (Models) are produced and distributed under rights to patents and intellectual property licensed from various institutions. Taconic grants to each purchaser a right under Taconic?s rights in such licensed patents and intellectual property to use the purchased Model in consideration of purchasers' acknowledgement of and agreement to the Terms and Conditions of Sale and the following terms of use:

• Title to these Models and biological materials derived from them remains with Taconic Farms, Inc.
• The Models will be used for research purposes only.
• The Models will not be bred except to obtain embryos or fetuses required for research purposes unless the purchaser maintains a Research Crossbreeding Agreement (PDF File: 75.5 KB) with Taconic Farms, Inc.
• The Models and biological materials derived from them will not be distributed to third parties or used for commercial purposes.

Health Reports/Production Sites

View the production locations(s) and the accompanying current IHMS^TM Health Report and Taconic Health Standard for this model

Price Table

7353 (in U.S. Dollars)

2010 HRN™ European Prices

Contact Us or call:

• 888.TACONIC (822.6642)
• Fax: 518.697.3910
• +45 70 23 04 05 (Europe)