Humanized Knock In with Optional Conditional Knock Out

Conditional Humanization combines the benefits of Humanization with Conditional approaches. This is achieved by first replacing the murine gene with its human orthologue. In addition, you have the option to also delete the humanized gene in a ubiquitous, spatial or temporal manner. This allows for in vivo testing of compounds and antibodies against human proteins in the mouse and comparing the effect against the null allele. All lines are generated using our extremely reliable ES cells on a C57BL/6 background. We also routinely delete the selection marker to avoid any potential hypomorphic effects..

Generation of Conditional Humanization consists of the following steps:

1. Targeting Vector Design & Construction
2. Targeted C57BL/6 ES cells
3. Generation of mice heterozygous for the conditional humanized Knock in

Expected timeline: 44 WEEKS

Taconic has incorporated rigorous quality control and documentation of different mouse production steps using project management software and the Laboratory Information Management System (LIMS). A designated Taconic scientific project manager will ensure optimal communication and strict adherence to the timeline and deliverables throughout the project. Taconic will provide a detailed Milestone Documentation after each of the steps mentioned above.

Upon generation of the line at Taconic, we will coordinate the delivery and further breeding of your animals to produce the research cohorts you want at the health status you need.

Learn more about our Cohort Production Packages for Conditional humanization Models.