Humanization

Humanization allows for in vivo testing of compounds and antibodies against human proteins in the mouse. This is achieved by the replacement of the murine gene with the human counterpart in a single step, placing the gene in the same transcriptional context as the murine gene. Depending on the necessity, replacements can be made ranging from single nucleotides to several kb of genomic DNA. All lines are generated using our extremely reliable ES cells on a C57BL/6 background. We also routinely delete the selection marker to avoid any potential hypomorphic effects.

Generation of Humanized models consists of the following steps:

1. Targeting Vector Design & Construction
2. Targeted C57BL/6 ES cells
3. Generation of mice heterozygous for the humanized Knock in

Expected timeline: 42 WEEKS

Taconic has incorporated rigorous quality control and documentation of different mouse production steps using project management software and the Laboratory Information Management System (LIMS). A designated Taconic scientific project manager will ensure optimal communication and strict adherence to the timeline and deliverables throughout the project. Taconic will provide a detailed Milestone Documentation after each of the steps mentioned above.

Upon generation of the line at Taconic, we will coordinate the delivery and further breeding of your animals to produce the research cohorts you want at the health status you need.

Learn more about our Cohort Production Packages for Humanized Models.