Constitutive Knock In

A Constitutive Knock In is the introduction of a mutation and/or reporter gene to follow gene function and/or expression. This method can also be combined with conditional® approaches, saving time and resources. We have developed a range of multi-purpose vectors that allow the generation of a constitutive KI mouse and then subsequently a conditional® mouse without the necessity of re-making the vector or re-targeting the ES cells or new chimeras. All lines are generated using our extremely reliable ES cells on a C57BL/6 background. We also routinely delete the selection marker to avoid any potential hypomorphic effects.

Generation of a Constitutive Knock In consists of the following steps:

1. Targeting Vector Design & Construction
2. Targeted C57BL/6 ES cells
3. Generation of heterozygous mice for the constitutive Knock in

Expected timeline: 40 WEEKS

Taconic has incorporated rigorous quality control and documentation of different mouse production steps using project management software and the Laboratory Information Management System (LIMS). A designated Taconic scientific project manager will ensure optimal communication and strict adherence to the timeline and deliverables throughout the project. Taconic will provide a detailed Milestone Documentation after each of the steps mentioned above.

Upon generation of the line at Taconic, we will coordinate the delivery and further breeding of your animals to produce the research cohorts you want at the health status you need.

Learn more about our Cohort Production Packages for Constitutive Knock In Models.