Constitutive Knock Out

A Constitutive Knock Out will eliminate the function of the targeted gene in all cells throughout development and adulthood. All lines can be generated directly on an inbred C57BL/6 background. We routinely delete the selection marker to avoid any potential hypomorphic effects.

Generation of a Constitutive Knock Out consists of the following major steps:

1. Targeting Vector Design & Construction
2. Targeted C57BL/6 ES cells
3. Generation of mice heterozygous for the constitutive Knock out

Expected timeline: 38 WEEKS

Taconic has incorporated rigorous quality control and documentation of different mouse production steps using project management software and Laboratory Information Management System (LIMS). A designated Taconic scientific project manager will ensure optimal communication and strict adherence to the timeline and deliverables throughout the project. Taconic will provide a detailed Milestone Documentation after each of the steps mentioned above.

Upon generation of the line at Taconic, we will coordinate the delivery and further breeding of your animals to produce the research cohorts you want at the health status you need.

Learn more about our Cohort Production Packages for Constitutive Knockout Models.