The Pirc rat was developed by William F. Dove and James Amos-Landgraf, et al. at the University of Wisconsin School of Medicine and Public Health. The model was created through N-ethyl-N-nitrosourea (ENU) treatment of F344/NTac males and screening of their progeny to identify the Pirc founder, which harbored a point mutation at Apc nucleotide 3409. This point mutation created a stop codon at codon 1137 of the gene. The founder was backcrossed at least 7 generations to the wild type F344/NTac strain to eliminate other possible ENU-induced mutations. Taconic received stock in July 2008 from the Dove laboratory. The line was embryo transfer derived, and the colony was maintained through breeding of heterozygotes to wild types.