Stat1

Constitutive Knock Out

Stat1 Constitutive Knock Out Mouse Model

129S6 Background

  • Model #
  • Genotype
  • Nomenclature
  • 2045-F
    ko/ko
    129S6/SvEv-Stat1tm1Rds
  • 2045-M
    ko/ko
    129S6/SvEv-Stat1tm1Rds
  • Contains a homozygous disruption of the Stat1 gene and complete lack of functional STAT1 proteins
  • The JAK-STAT signaling pathway has been implicated in mediating biologic responses induced by many cytokines
  • Homozygous mice develop normally
  • Deficient immune cell response to alpha(α) and gamma(γ) interferons
  • Extreme susceptibility to infections by microbial pathogens and viruses
  • Could be used as sentinel animals for evaluating the health status of a mouse colony
  • Accelerated and amplified development of chemically-induced and spontaneous tumors
  • Useful in determining the role of a variety of cytokines in immune responses, the role of STAT1 protein in mediating interferon-dependent responses, and the roles of tumor cells and immune cells in mediating tumor cell destruction

Genetic Background:

129S6 Background

Origin:

The Stat1 mouse was developed in the laboratory of Robert Schreiber at the Washington University School of Medicine. The model was created by targeting the Stat1 gene in GS-1 ES cells and injecting the targeted cells into blastocysts. Heterozygotes for the mutation were produced from the chimeras and intercrossed to generate homozygotes. Taconic received stock in 1997. The mice were embryo transfer derived. The original Stat1 model (000679-M) differed from the Taconic 129S6 at two biochemical loci, Pgm1 and Gpi1. In January 2002, the original Stat1 line was backcrossed to the Taconic 129S6/SvEvTac, and animals were selected to match the Pgm1c and Gpi1b alleles of the 129S6. The selected heterozygotes were intercrossed to homozygosity. The line is maintained through incrossing of homozygous mice. This improvement makes the 129S6/SvEvTac a more perfectly matched control for the Stat1 model.


Genetics:

Wild type for Nnt mutation


Color:

White-Bellied Agouti

Species:

Mouse

Initial Publication:

Meraz MA, White JM, Sheehan KC, Bach EA, Rodig SJ, Dighe AS, Kaplan DH, Riley JK, Greenlund AC, Campbell D, Carver-Moore K, DuBois RN, Clark R, Aguet M, Schreiber RD. (1996) Targeted disruption of the Stat1 gene in mice reveals unexpected physiologic specificity in the JAK-STAT signaling pathway. Cell, 84(3):431-442.



Conditions of Use for Taconic Transgenic Models™
Taconic Transgenic Models™ (Models) are produced and distributed under rights to patents and intellectual property licensed from various institutions. Taconic sells the Models to purchasers, grants to each purchaser a right under Taconic's rights in such licensed patents and intellectual property to use the purchased Model in consideration of purchasers' acknowledgement of and agreement to the Terms and Conditions of Sale and the following terms of use:
  • Title to these Models and biological materials derived from them remains with Taconic Biosciences, Inc.
  • The Models will be used for research purposes only.
  • The Models will not be bred except to obtain embryos or fetuses required for research purposes
  • The Models and biological materials derived from them will not be distributed to third parties or used for commercial purposes.