Rag2 - Model RAGN12

Constitutive Knock Out

Rag2 (Model RAGN12) Constitutive Knock Out Mouse Model

C57BL/6NTac Background

  • Model #
  • Genotype
  • Nomenclature
  • RAGN12-F
    ko/ko
    B6.129S6-Rag2tm1Fwa N12
  • RAGN12-M
    ko/ko
    B6.129S6-Rag2tm1Fwa N12
  • Contains a disruption of the recombination activating gene 2 (Rag2)
  • Homozygous mice exhibit total inability to initiate V(D)J rearrangement and fail to generate mature T or B lymphocytes. Otherwise, the Rag2 mouse has apparently normal hematopoiesis
  • Useful for vaccine development, transplantation studies and hematopoiesis research. Through blastocyst complementation assays, can be useful in evaluating the function of specific genes in the differentiation of lymphocytes
  • Carries the H2b haplotype

Genetic Background:

C57BL/6 Background

Origin:

The Rag2 mouse was developed in the laboratory of Frederick W. Alt at Columbia University. The model was created by targeting the Rag2 gene in CCE ES cells and injecting the targeted cells into blastocysts. This line originated from model RAG2-M on a 129S6/SvEv background. The mice were backcrossed twelve generations (N12) to a C57BL/6NTac inbred background. Heterozygotes were intercrossed to generate homozygous targeted mutation mice. The line is maintained by incrossing homozygous mice.


Genetics:

Wild type for Nnt mutation


Color:

Black

Species:

Mouse

Initial Publication:

Shinkai, Y, Rathbun, G, Lam, KP, Oltz, EM, Stewart, V, Mendelsohn, M, Charron, J, Datta, M, Young, F, Stall, AM, and Alt, FW. (1992) RAG-2-Deficient Mice Lack Mature Lymphocytes Owing to Inability to Initiate V(D)J Rearrangement, Cell, 68: 855-867.



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Taconic Transgenic Models™ (Models) are produced and distributed under rights to patents and intellectual property licensed from various institutions. Taconic sells the Models to purchasers, grants to each purchaser a right under Taconic's rights in such licensed patents and intellectual property to use the purchased Model in consideration of purchasers' acknowledgement of and agreement to the Terms and Conditions of Sale and the following terms of use:
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  • The Models will be used for research purposes only.
  • The Models will not be bred except to obtain embryos or fetuses required for research purposes
  • The Models and biological materials derived from them will not be distributed to third parties or used for commercial purposes.