The Pfp/Rag2 mouse was developed by crossbreeding the Pfp targeted mutation mouse (model PFPN12) and the Rag2 targeted mutation mouse (model RAGN12) at Taconic and breeding to homozygosity for both genes. This model was backcrossed twelve generations (N12) to C57BL/6NTac. The colony was maintained through homozygous matings.
Wild type for Nnt mutation
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Walsh CM, Matloubian M, Liu C, Ueda R, Kurahara CG, Christensen JL, Huang MTF, Young JD, Ahmed R, and Clark WR. (1994) Immune function in mice lacking the perforin gene. Proc Natl Acad Sci USA, 91(23): 10854-10858.