Oct1/2

Constitutive Knock Out

Oct1/2 Constitutive Knock Out Mouse Model

FVB Background

  • Model #
  • Genotype
  • Nomenclature
  • 6622-F
    ko/ko;ko/ko
    FVB.129P2-Slc22a1tm1Ahs  Slc22a2tm1Ahs N7
  • 6622-M
    ko/ko;ko/ko
    FVB.129P2-Slc22a1tm1Ahs  Slc22a2tm1Ahs N7
  • This double targeted mutation strain carries a disruption of organic cation transporter genes Slc22a1 and Slc22a2
  • Useful for studies of drug transport in the liver, intestine and kidneys
  • OCT1 and OCT2 determine renal secretion of small organic cations

Genetic Background:

FVB Background

Origin:

The Oct1/2 mouse was developed in the laboratory of Alfred Schinkel of the Netherlands Cancer Institute. The model was created through sequential targeting of the Slc22a1 and Slc22a2 genes in 129/Ola-derived E14 ES cells and injecting the targeted cells into C57BL/6 blastocysts. Resultant chimeras were backcrossed to FVB/N for seven generations (N7). Taconic received stock in 2006, and the line was embryo transfer derived. The colony is maintained by mating doubly homozygous mice.


Color:

Albino

Species:

Mouse

Initial Publication:

Jonker JW, Wagenaar E, Mol CA, Buitelaar M, Koepsell H, Smit JW, Schinkel AH. (2001) Reduced hepatic uptake and intestinal excretion of organic cations in mice with a targeted disruption of the organic cation transporter 1 (Oct1 [Slc22a1]) gene. Mol Cell Biol 21(16): 5471-5477.

Jonker JW, Wagenaar E, Van Eijl S, Schinkel AH. (2003) Deficiency in the organic cation transporters 1 and 2 (Oct1/2 [Slc22a1/Slc22a2]) in mice abolishes renal secretion of organic cations. Mol Cell Biol 23(21):7902-7908.



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Taconic Transgenic Models™ (Models) are produced and distributed under rights to patents and intellectual property licensed from various institutions. Taconic sells the Models to purchasers, grants to each purchaser a right under Taconic's rights in such licensed patents and intellectual property to use the purchased Model in consideration of purchasers' acknowledgement of and agreement to the Terms and Conditions of Sale and the following terms of use:
  • Title to these Models and biological materials derived from them remains with Taconic Biosciences, Inc.
  • The Models will be used for research purposes only.
  • The Models will not be bred except to obtain embryos or fetuses required for research purposes
  • The Models and biological materials derived from them will not be distributed to third parties or used for commercial purposes.