The Oct1/2 mouse was developed in the laboratory of Alfred Schinkel of the Netherlands Cancer Institute. The model was created through sequential targeting of the Slc22a1 and Slc22a2 genes in 129/Ola-derived E14 ES cells and injecting the targeted cells into C57BL/6 blastocysts. Resultant chimeras were backcrossed to FVB/N for seven generations (N7). Taconic received stock in 2006, and the line was embryo transfer derived. The colony is maintained by mating doubly homozygous mice.
Jonker JW, Wagenaar E, Mol CA, Buitelaar M, Koepsell H, Smit JW, Schinkel AH. (2001) Reduced hepatic uptake and intestinal excretion of organic cations in mice with a targeted disruption of the organic cation transporter 1 (Oct1 [Slc22a1]) gene. Mol Cell Biol 21(16): 5471-5477.
Jonker JW, Wagenaar E, Van Eijl S, Schinkel AH. (2003) Deficiency in the organic cation transporters 1 and 2 (Oct1/2 [Slc22a1/Slc22a2]) in mice abolishes renal secretion of organic cations. Mol Cell Biol 23(21):7902-7908.