Oatp1a/1b Cluster Knockout Mouse

Constitutive Knock Out

Oatp1a/1b Cluster Constitutive Knock Out Mouse Model

FVB Background

  • Model #
  • Genotype
  • Nomenclature
  • 10707-F
    ko/ko
    FVB.129P2-Del(Slco1b2-Slco1a5)1Ahs
  • 10707-M
    ko/ko
    FVB.129P2-Del(Slco1b2-Slco1a5)1Ahs
  • This model carries a deletion of all five established Slco1a and 1b and two predicted Slco1a-like mouse genes.
  • Contains a cre-mediated deletion of the following established genes in the Slco1a/1b cluster: Slco1a1, Slco1a4, Slco1a5, Slco1a6 and Slco1b2.
  • OATPs facilitate sodium-independent uptake transport of a wide variety of organic endo and exogenous compounds, such as bile acids, steroid and thyroid hormones and their conjugates, and numerous drugs and toxins.
  • Useful in dissecting the contribution of the Slco1a/1b family in these processes.

Genetic Background:

FVB/N Background

Origin:

The Oatp1a/1b Cluster Knockout Mouse was developed in the laboratory of Alfred Schinkel of the Netherlands Cancer Institute in 2010. The model was generated by insertion?of?loxP?sites?into the Slco1a5 and Slco1b2 genes at?both?ends?of?the?Slco1a/1b?gene?cluster in E14 embryonic stem cells derived from 129P2/OlaHsd mice, followed by Cre-mediated?deletion and injecting the targeted cells into C57BL/6J blastocysts. Resultant chimeras were backcrossed to FVB/N mice for at least 7 generations (N7). Taconic received stock in 2010. The mice were derived by embryo transfer and are maintained by incrossing of homozygous mice.


Genetics:

Wild type for Nnt mutation; carries Pde6brd1 mutation


Color:

Albino

Species:

Mouse

Initial Publication:

van de Steeg E, Wagenaar E, van der Kruijssen CM, Burggraaff JE, de Waart DR, Elferink RP, Kenworthy KE, Schinkel AH (2010). Organic anion transporting polypeptide 1a/1b-knockout mice provide insights into hepatic handling of bilirubin, bile acids, and drugs. J. Clin. Invest. 120(8): 2942-52.



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Taconic Transgenic Models™ (Models) are produced and distributed under rights to patents and intellectual property licensed from various institutions. Taconic sells the Models to purchasers, grants to each purchaser a right under Taconic's rights in such licensed patents and intellectual property to use the purchased Model in consideration of purchasers' acknowledgement of and agreement to the Terms and Conditions of Sale and the following terms of use:
  • Title to these Models and biological materials derived from them remains with Taconic Biosciences, Inc.
  • The Models will be used for research purposes only.
  • The Models will not be bred except to obtain embryos or fetuses required for research purposes
  • The Models and biological materials derived from them will not be distributed to third parties or used for commercial purposes.