The Oatp1a/1b Cluster Knockout Mouse was developed in the laboratory of Alfred Schinkel of the Netherlands Cancer Institute in 2010. The model was generated by insertion?of?loxP?sites?into the Slco1a5 and Slco1b2 genes at?both?ends?of?the?Slco1a/1b?gene?cluster in E14 embryonic stem cells derived from 129P2/OlaHsd mice, followed by Cre-mediated?deletion and injecting the targeted cells into C57BL/6J blastocysts. Resultant chimeras were backcrossed to FVB/N mice for at least 7 generations (N7). Taconic received stock in 2010. The mice were derived by embryo transfer and are maintained by incrossing of homozygous mice.
Wild type for Nnt mutation; carries Pde6brd1 mutation
van de Steeg E, Wagenaar E, van der Kruijssen CM, Burggraaff JE, de Waart DR, Elferink RP, Kenworthy KE, Schinkel AH (2010). Organic anion transporting polypeptide 1a/1b-knockout mice provide insights into hepatic handling of bilirubin, bile acids, and drugs. J. Clin. Invest. 120(8): 2942-52.