Mdr1a

Constitutive Knock Out

Mdr1a Constitutive Knock Out Mouse Model

FVB Background

  • Model #
  • Genotype
  • Nomenclature
  • MDR1A-F
    ko/ko
    FVB.129P2-Abcb1atm1Bor N7
  • MDR1A-M
    ko/ko
    FVB.129P2-Abcb1atm1Bor N7
  • Carries a disruption of multi-drug resistance genes Abcb1a (ATP-binding cassette, sub-family B (MDR/TAP), member 1A, a.k.a. Mdr1a) encoding p-glycoprotein 3
  • Mdr1a protein is expressed actively in the intestine and at the blood-brain and blood-testis barriers
  • p-glycoprotein 3 actively extrudes a wide range of drugs from cells and can confer multi-drug resistance to tumor cells
  • Lack the protective function of p-glycoprotein 3 and exhibits a functional deficiency in the blood-brain barrier
  • Useful in neurotoxicology, drug transport, chemotherapy, multi-drug resistance and oral bioavailability research
  • Susceptible to developing a severe, spontaneous intestinal inflammation phenotypically similiar to human inflammatory bowel disease (IBD)

Genetic Background:

FVB Background

Origin:

The Mdr1a mouse was developed in the laboratory of Alfred Schinkel of the Netherlands Cancer Institute. The model was created by targeting the Abcb1a gene in E14 ES cells. Resultant chimeras were backcrossed to FVB for seven (N7) generations. Taconic received stock in 1994 and derived the line by Caesarean transfer. The colony is maintained by incrossing homozygous mice.


Genetics:

Wildtype for Nnt mutation; carries Pde6brd1 mutation


Color:

Albino

Species:

Mouse

Initial Publication:

Schinkel AH, Smit JJM, van Tellingen O, Beijnen JH, Wagenaar E, van Deetmer L, Mol CAAM, van der Valk MA, Robanus-Maandag BC, te Tiele HPJ, Berns AJM, Borst P. (1994) Disruption of the Mouse mdr1a P-Glycoprotein Gene Leads to a Deficiency in the Blood Brain Barrier and to Increased Sensitivity to Drugs. Cell, 77:491-502.



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Taconic Transgenic Models™ (Models) are produced and distributed under rights to patents and intellectual property licensed from various institutions. Taconic sells the Models to purchasers, grants to each purchaser a right under Taconic's rights in such licensed patents and intellectual property to use the purchased Model in consideration of purchasers' acknowledgement of and agreement to the Terms and Conditions of Sale and the following terms of use:
  • Title to these Models and biological materials derived from them remains with Taconic Biosciences, Inc.
  • The Models will be used for research purposes only.
  • The Models will not be bred except to obtain embryos or fetuses required for research purposes
  • The Models and biological materials derived from them will not be distributed to third parties or used for commercial purposes.