The Df(h22q11)/+ mouse line was developed for H. Lundbeck A/S, by Taconic Biosciences GmbH (Cologne, Germany), by deletion of the human h22q11.2 orthologous genomic region on mouse chromosome 16. Two targeting vectors were generated using bacterial artificial chromosome clones from the C57BL/6J RPCI-23 library and transfected into TaconicArtemis C57BL/6N Tac embryonic stem cell line. The first vector introduced a loxP site upstream of the Dgcr2 gene. The second vector introduced a loxP site downstream of the Hira gene. Homologous recombinant clones were isolated and the 1.13 megabase region on mouse chromosome 16 between the loxP sites was removed using in vitro Cre-mediated recombination. Hemizygotic embryonic stem cells were injected into blastocysts isolated from impregnated BALB/c female mice and transferred to pseudopregnant NMRI female mice. Chimeric male pups were selected by coat color and mated with wild-type C57BL/6 female mice. Finally, a chimera with germline transmission was selected for expansion breeding. The colony is maintained by mating wild-type C57BL/6N mice with hemizygous Df(h22q11)/+ mice.
Didriksen M, Fejgin K, Nilsson SR, Birknow MR, Grayton HM, Larsen PH, Lauridsen JB, Nielsen V, Celada P, Santana N, Kallunki P, Christensen KV, Werge TM, Stensbøl TB, Egebjerg J, Gastambide F, Artigas F, Bastlund JF, Nielsen J. (2016) Persistent gating deficit and increased sensitivity to NMDA receptor antagonism after puberty in a new mouse model of the human 22q11.2 microdeletion syndrome: a study in male mice. J Psychiatry Neurosci. 2016 Jul 7;41(5):150381.