HLA-A11

Random Transgenic

HLA-A11 Random Transgenic Mouse Model

CB6F1 Background

  • Model #
  • Genotype
  • Nomenclature
  • 9660-F
    tg/wt
    CB6F1-Tg(HLA-A*1101/H2-Kb)A11.01
  • 9660-M
    tg/wt
    CB6F1-Tg(HLA-A*1101/H2-Kb)A11.01
  • Carries a transgene consisting of fragments of the human HLA-A*1101 gene and mouse H2-Kb gene which encodes a chimeric class I molecule consisting of the human HLA-A11 leader, α1 and α2 domains ligated to the murine α3, transmembrane and cytoplasmic H2-Kb domains
  • Expresses the chimeric HLA-A11 class I molecule on the surface of T and B cells
  • Represents the human HLA-A3 supertype
  • Useful for infectious disease research, vaccine development and testing, safety and immunogenicity testing as well as research directed towards oncology and autoimmune disorders
  • Permits identification of epitopes restricted to the HLA-A11 supertype

Genetic Background:

CB6F1 Background

Origin:

The HLA-A11 model was generated by Pharmexa-Epimmune. The model was created by microinjecting a chimeric transgene combining a fragment of the cDNA clone of the human HLA-A*1101 gene that included leader sequence, α1 and α2 domains ligated to a fragment of the murine H2-Kb gene containing the α3, transmembrane and cytoplasmic domains. This transgene was microinjected into CxB6 zygotes. The resultant mice from Founder Line A11.01 were backcrossed six generations to C57BL/6J. Taconic received stock from Pharmexa-Epimmune in 2008. The line was embryo transfer derived, backcrossed one generation to C57BL/6NTac and then intracrossed to homozygosity. In the production colony, wild type BALB/cAnNTac females are bred to homozygous males on the C57BL/6 background to produce CB6F1 pups.


Color:

Black Agouti

Species:

Mouse

Initial Publication:

Alexander J, Oseroff C, Sidney J, Wentworth P, Keogh E, Hermanson G, Chisari FV, Kubo RT, Grey HM, Sette A. (1997) Derivation of HLA-A11/Kb transgenic mice: functional CTL repertoire and recognition of human A11-restricted CTL epitopes. J Immunol 159(10):4753-61. Erratum in: J Immunol 1999 162(5):3104.



Conditions of Use for Taconic Transgenic Models™
Taconic Transgenic Models™ (Models) are produced and distributed under rights to patents and intellectual property licensed from various institutions. Taconic sells the Models to purchasers, grants to each purchaser a right under Taconic's rights in such licensed patents and intellectual property to use the purchased Model in consideration of purchasers' acknowledgement of and agreement to the Terms and Conditions of Sale and the following terms of use:
  • Title to these Models and biological materials derived from them remains with Taconic Biosciences, Inc.
  • The Models will be used for research purposes only.
  • The Models will not be bred except to obtain embryos or fetuses required for research purposes
  • The Models and biological materials derived from them will not be distributed to third parties or used for commercial purposes.