HLA-A1

Random Transgenic

HLA-A1 Random Transgenic Mouse Model

CB6F1 Background

  • Model #
  • Genotype
  • Nomenclature
  • 9662-F
    tg/wt
    CB6F1-Tg(HLA-A*0101/H2-Kb)A1.01
  • 9662-M
    tg/wt
    CB6F1-Tg(HLA-A*0101/H2-Kb)A1.01
  • 9662-F
    wt/wt
    CB6F1-Tg(HLA-A*0101/H2-Kb)A1.01
  • 9662-M
    wt/wt
    CB6F1-Tg(HLA-A*0101/H2-Kb)A1.01
  • Carries a transgene consisting of fragments of the human HLA-A*0101 gene and mouse H2-Kb gene which encodes a chimeric class I molecule consisting of the human HLA-A1 leader, α1 and α2 domains ligated to the murine α3, transmembrane and cytoplasmic H2-Kb domains
  • Expresses the chimeric HLA-A1 class I molecule on the surface of T and B cells
  • Represents the human HLA-A1 supertype
  • Useful for infectious disease research, vaccine development and testing, safety and immunogenicity testing as well as research directed towards oncology and autoimmune disorders
  • Permits identification of epitopes restricted to the HLA-A1 supertype
Orders by weight: Taconic cannot accept orders by weight for this model. Please note that shipments may contain animals with a larger weight variation.

Origin:

The HLA-A1 model was generated by Pharmexa-Epimmune. The model was created by microinjecting a chimeric transgene combining a fragment of the genomic clone of the human HLA-A*0101 gene that included the leader sequence, α1 and α2 domains ligated to a fragment of the murine H2-Kb gene containing the α3, transmembrane and cytoplasmic domains, all under the control of the murine H2-Kb promoter. This transgene was microinjected into C57BL/6 zygotes. The resultant mice from Founder Line A1.01 were on an inbred C57BL/6 background. The line was maintained by breeding hemizygotes to wild type C57BL/6NTac mice for 10 generations. Taconic received stock from Pharmexa-Epimmune in 2008. The line was embryo transfer derived by breeding C57BL/6NTac wild type females to hemizygous males. The foundation and expansion colonies are maintained through breeding of hemizygous males to wild type C57BL/6NTac females. In the production colony, wild type BALB/cAnNTac females are bred to hemizygous males on the C57BL/6NTac background to produce CB6F1 pups.


Color:

Black Agouti

Species:

Mouse

Initial Publication:

Depla E, Van der Aa A, Livingston BD, Crimi C, Allosery K, De Brabandere V, Krakover J, Murthy S, Huang M, Power S, Babé L, Dahlberg C, McKinney D, Sette A, Southwood S, Philip R, Newman MJ, Meheus L. (2008) Rational design of a multiepitope vaccine encoding T-lymphocyte epitopes for treatment of chronic hepatitis B virus infections. J Virol. 82(1):435-50.

 



Conditions of Use for Taconic Transgenic Models™
Taconic Transgenic Models™ (Models) are produced and distributed under rights to patents and intellectual property licensed from various institutions. Taconic sells the Models to purchasers, grants to each purchaser a right under Taconic's rights in such licensed patents and intellectual property to use the purchased Model in consideration of purchasers' acknowledgement of and agreement to the Terms and Conditions of Sale and the following terms of use:
  • Title to these Models and biological materials derived from them remains with Taconic Biosciences, Inc.
  • The Models will be used for research purposes only.
  • The Models will not be bred except to obtain embryos or fetuses required for research purposes
  • The Models and biological materials derived from them will not be distributed to third parties or used for commercial purposes.