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Contains a disruption of the beta-2-microglobulin gene
Deficient in MHC Class I molecule expression
Exhibits depletion of CD8+ T cells
Lack the NK1.1+ CD4+ T-cell population as well, because of dependence on MHC Class 1b molecule CD1 for development
Useful in transplantation, gene therapy, and immunological disease research
Taconic cannot accept orders by weight for this model. Please note that shipments may contain animals with a larger weight variation.
Genetic Background: C57BL/6 Background
Origin: The B2m mouse was developed in the laboratory of Rudolf Jaenisch at the Whitehead Institute. The model was created by targeting the B2m gene in D3 ES cells and injecting the targeted cells into C57BL/6J blastocysts. Resultant chimeras were backcrossed to C57BL/6 for five generations (N5). GenPharm International received stock from the Jaenisch lab in 1991. Taconic received stock in February 1994. The mice were then backcrossed seven generations (N12) to C57BL/6NTac and derived by embryo transfer. Heterozygotes were intercrossed to homozygosity. The colony is maintained through incrossing of homozygotes.
Genetics: Wild type for Nnt mutation
Zijlstra M, Bix M, Simister NE, Loring JM, Raulet DH, Jaenisch R. (1990) β2-Microglobulin deficient mice lack CD4-8+ cytolytic T cells
, Nature, 344(6268):742-746