Targeted Replacement

APOE3 Targeted Replacement Mouse Model

C57BL/6 Background

  • Model #
  • Genotype
  • Nomenclature
  • 1548-F
    B6.129P2-Apoetm2(APOE*3)Mae N8
  • 1548-M
    B6.129P2-Apoetm2(APOE*3)Mae N8
  • Homozygous for a human APOE3 gene targeted replacement of the endogenous mouse Apoe gene
  • Expresses human apolipoprotein E3 isoform under the control of the murine Apoe regulatory sequences
  • ApoE is a plasma protein involved in cholesterol transport, with three human isoforms (E2, E3, and E4) that have been associated with atherosclerosis and Alzheimer's Disease (AD)
  • E3 is the most common isoform, expressed by almost 80% of the human population
  • On a normal diet, this model has normal plasma cholesterol and triglyceride levels, but altered relative quantities of different plasma lipoprotein particles, and delayed clearance of vLDL particles
  • On a high-fat diet, develops abnormal serum lipid profiles and atherosclerotic plaques
  • Exhibits an increased risk of atherosclerosis and hypercholesterolemia compared with wild type mice on a high fat diet, but not on a normal diet
  • Useful for studying the role of human APOE polymorphism in atherosclerosis, lipid metabolism and Alzheimer's disease

Genetic Background:

C57BL/6 Background


The APOE3 targeted replacement mouse was developed in the laboratory of Nobuya Maeda at the University of North Carolina. The model was created by targeting the murine Apoe gene for replacement with the human APOE3 allele in E14TG2a ES cells and injecting the targeted cells into blastocysts. Resultant chimeras were backcrossed to C57BL/6 for seven generations (N7). Taconic received stock in 2000. The mice were backcrossed once more (N8) and embryo transfer derived. The colony is maintained through incrossing of homozygotes.


Carries Nnt mutation





Initial Publication:

Sullivan PM, Mezdour H, Aratani Y, Knouff C, Najib J, Reddick RL, Quarfordt SH, Maeda N. (1997) Targeted replacement of the mouse apolipoprotein E gene with the common human APOE3 allele enhances diet-induced hypercholesterolemia and atherosclerosis. J Biol Chem, 272(9):17972-80.

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Taconic Transgenic Models™ (Models) are produced and distributed under rights to patents and intellectual property licensed from various institutions. Taconic sells the Models to purchasers, grants to each purchaser a right under Taconic's rights in such licensed patents and intellectual property to use the purchased Model in consideration of purchasers' acknowledgement of and agreement to the Terms and Conditions of Sale and the following terms of use:
  • Title to these Models and biological materials derived from them remains with Taconic Biosciences, Inc.
  • The Models will be used for research purposes only.
  • The Models will not be bred except to obtain embryos or fetuses required for research purposes
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