Abb (H2-Ab1) - Model 4026

Constitutive Knock Out

Abb (H2-Ab1) 4026 Constitutive Knock Out Mouse Model
EZcohort™ Models
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B6.SJL Background

  • Model #
  • Genotype
  • Nomenclature
  • 4026-F
    ko/ko
    B6.SJL(129)-Ptprca H2-Ab1tm1Gru
  • 4026-M
    ko/ko
    B6.SJL(129)-Ptprca H2-Ab1tm1Gru
  • Contains a disruption of the H2-Ab1 gene
  • Expresses no A or E MHC class II molecules and therefore lacks most CD4+ T cells
  • Useful in transplantation, gene therapy and immunological disease research
  • The Ptprca marker on hematopoietic cells of this congenic strain allows for their identification in immunological adoptive transfer experiments

Genetic Background:

C57BL/6-Ptprc a Background

Origin:

The Abb mouse was developed in the laboratory of Michael J. Grusby et al. of the Harvard School of Public Health. The model was created by targeting the H2-Ab1 gene in ES-D3 cells and injecting the targeted ES cells into C57BL/6 blastocysts. Resultant chimeras were backcrossed to C57BL/6 for 7 generations (N7), then backcrossed four generations (N4) to B6.SJL- Ptprca. NIAID received stock in 1994, and Taconic received stock in 1995 for the NIAID Transgenic Mouse Repository. The mice were then derived via backcross to B6.SJL-Ptprca. The mice were backcrossed once more to B6.SJL-Ptprca (N5), then intercrossed to homozygosity for both Abb and Ptprca. The mice were derived by embryo transfer again in 1997 (N6). The line was maintained by incrossing homozygous mice.


Genetics:

Wild type for Nnt mutation, carries Pde6brd1 mutation


Color:

Black

Species:

Mouse

Initial Publication:

Grusby MJ, Johnson RS, Papaioannou VE, Glimcher LH. (1991) Depletion of CD4+ T-Cells in Major Histocompatibility Complex Class II - Deficient Mice. Science, 253(5026):1417-1420.



Conditions of Use for EZcohort™ Models
EZcohort™ models are produced and distributed under rights to patents and intellectual property licensed from various institutions. Taconic Biosciences, Inc. (Taconic) sells EZcohort™ models to purchasers, grants to each purchaser a right under Taconic's rights in such licensed patents and intellectual property to use the purchased EZcohort™ models in consideration of purchasers' acknowledgement of and agreement to Taconic's Terms and Conditions for the Sale of Products and the following conditions of use:

  • Title to EZcohort™ models and biological materials derived from them remains with Taconic.
  • EZcohort™ models will be used for research purposes only.
  • EZcohort™ models and biological materials derived from them will not be distributed to third parties or used for commercial purposes.
  • Breeders from deliverables can be used for propagation of EZcohort™ models or to cross to other strains.
  • Continuation of breeding and crossbreeding of EZcohort™ models outside Taconicis permitted for a term of three years post-purchase of the EZcohort™ models and is subject to annual confirmation of these EZcohort™ Conditions of Use.

Continuation of breeding and crossbreeding after the three year term will require renewal of the EZcohort™ Conditions of Use and payment of a fee.